|Application ||WB, IHC-P, IF, IP|
|Reactivity||Human, Mouse, Rat, Hamster, Guinea Pig|
|Calculated MW||49408 Da|
|Dilution||IHC-P (10 µg/ml),|
|Other Names||WIPI2, ATG18B, Atg21, DKFZP434J154, WIPI-2, WIPI49-like protein 2, CGI-50|
|Target/Specificity||Recognizes WIPI2, a mammalian ortholog of Atg18. WIPI2 is recruited to early autophagosomal structures and is required for their maturation into mature autophagosomes.|
|Reconstitution & Storage||PBS, 0.09% sodium azide. +4°C or -20°C, Avoid repeated freezing and thawing.|
|Precautions||WIPI2 Antibody (C-Terminus, clone 2A2) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Early component of the autophagy machinery being involved in formation of preautophagosomal structures and their maturation into mature phagosomes in response to phosphatidylinositol 3-phosphate (PtdIns3P). May bind PtdIns3P.|
|Cellular Location||Preautophagosomal structure membrane; Peripheral membrane protein; Cytoplasmic side Note=Localizes to omegasomes membranes which are endoplasmic reticulum connected strutures at the origin of preautophagosomal structures. Enriched at preautophagosomal structure membranes in response to PtdIns3P.|
|Tissue Location||Ubiquitously expressed (at protein level). Highly expressed in heart, skeletal muscle and pancreas Expression is down-regulated in pancreatic and in kidney tumors|
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Provided below are standard protocols that you may find useful for product applications.
Early component of the autophagy machinery being involved in formation of preautophagosomal structures and their maturation into mature phagosomes in response to phosphatidylinositol 3-phosphate (PtdIns3P). May bind PtdIns3P.
Proikas-Cezanne T.,et al.Oncogene 23:9314-9325(2004).
Lai C.-H.,et al.Genome Res. 10:703-713(2000).
Wiemann S.,et al.Genome Res. 11:422-435(2001).
Ebert L.,et al.Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).
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