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WIPI2 Antibody (C-Terminus, clone 2A2)
Mouse Monoclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-P, IF, IP |
|---|---|
| Primary Accession | Q9Y4P8 |
| Other Accession | 26100 |
| Reactivity | Human, Mouse, Rat, Hamster, Guinea Pig |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone Names | 2A2 |
| Calculated MW | 49408 Da |
| Dilution | IHC-P (10 µg/ml), |
| Gene ID | 26100 |
|---|---|
| Other Names | WIPI2, ATG18B, Atg21, DKFZP434J154, WIPI-2, WIPI49-like protein 2, CGI-50 |
| Target/Specificity | Recognizes WIPI2, a mammalian ortholog of Atg18. WIPI2 is recruited to early autophagosomal structures and is required for their maturation into mature autophagosomes. |
| Reconstitution & Storage | PBS, 0.09% sodium azide. +4°C or -20°C, Avoid repeated freezing and thawing. |
| Precautions | WIPI2 Antibody (C-Terminus, clone 2A2) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | WIPI2 (HGNC:32225) |
|---|---|
| Function | Component of the autophagy machinery that controls the major intracellular degradation process by which cytoplasmic materials are packaged into autophagosomes and delivered to lysosomes for degradation (PubMed:20505359, PubMed:28561066). Involved in an early step of the formation of preautophagosomal structures (PubMed:20505359, PubMed:28561066). Binds and is activated by phosphatidylinositol 3- phosphate (PtdIns3P) forming on membranes of the endoplasmic reticulum upon activation of the upstream ULK1 and PI3 kinases (PubMed:28561066). Mediates ER-isolation membranes contacts by interacting with the ULK1:RB1CC1 complex and PtdIns3P (PubMed:28890335). Once activated, WIPI2 recruits at phagophore assembly sites the ATG12-ATG5-ATG16L1 complex that directly controls the elongation of the nascent autophagosomal membrane (PubMed:20505359, PubMed:28561066). |
| Cellular Location | Preautophagosomal structure membrane; Peripheral membrane protein; Cytoplasmic side. Note=Localizes to omegasomes membranes which are endoplasmic reticulum connected structures at the origin of preautophagosomal structures. Enriched at preautophagosomal structure membranes in response to PtdIns3P. |
| Tissue Location | Ubiquitously expressed (at protein level). Highly expressed in heart, skeletal muscle and pancreas. Expression is down- regulated in pancreatic and in kidney tumors |
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Provided below are standard protocols that you may find useful for product applications.
Background
Early component of the autophagy machinery being involved in formation of preautophagosomal structures and their maturation into mature phagosomes in response to phosphatidylinositol 3-phosphate (PtdIns3P). May bind PtdIns3P.
References
Proikas-Cezanne T.,et al.Oncogene 23:9314-9325(2004).
Lai C.-H.,et al.Genome Res. 10:703-713(2000).
Wiemann S.,et al.Genome Res. 11:422-435(2001).
Ebert L.,et al.Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).
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