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>   home   >   Products   >   Primary Antibodies   >   DDX39B / UAP56 Antibody (clone 2E4)   

DDX39B / UAP56 Antibody (clone 2E4)

Mouse Monoclonal Antibody

     
  • IHC-P - DDX39B / UAP56 Antibody (clone 2E4) ALS17258
    Human Skin: Formalin-Fixed, Paraffin-Embedded (FFPE)
  • IF - DDX39B / UAP56 Antibody (clone 2E4) ALS17258
    Anti-BAT1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently...
  • WB - DDX39B / UAP56 Antibody (clone 2E4) ALS17258
    HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BAT1...
  • WB - DDX39B / UAP56 Antibody (clone 2E4) ALS17258
    Western blot of extracts (35 ug) from 9 different cell lines by using anti-BAT1 monoclonal...
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IF
Primary Accession Q13838
Other Accession 7919
Reactivity Human, Rat, Dog
Host Mouse
Clonality Monoclonal
Isotype IgG2b
Clone Names 2E4
Calculated MW 48991 Da
Dilution IF (1:100), IHC-P (1:100), WB (1:500),
Additional Information
Gene ID 7919
Other Names DDX39B, BAT1, DEAD box protein UAP56, HLA-B associated transcript 1, UAP56, ATP-dependent RNA helicase p47, D6S81E, Spliceosome RNA helicase BAT1
Target/Specificity Human DDX39B / UAP56
Reconstitution & Storage PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide. Store at -20°C. Minimize freezing and thawing.
PrecautionsDDX39B / UAP56 Antibody (clone 2E4) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name DDX39B
Synonyms BAT1, UAP56
Function Involved in nuclear export of spliced and unspliced mRNA. Assembling component of the TREX complex which is thought to couple mRNA transcription, processing and nuclear export, and specifically associates with spliced mRNA and not with unspliced pre-mRNA. TREX is recruited to spliced mRNAs by a transcription- independent mechanism, binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export to the cytoplasm via the TAP/NFX1 pathway. May undergo several rounds of ATP hydrolysis during assembly of TREX to drive subsequent loading of components such as ALYREF/THOC and CHTOP onto mRNA. The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production. Also associates with pre-mRNA independent of ALYREF/THOC4 and the THO complex. Involved in the nuclear export of intronless mRNA; the ATP-bound form is proposed to recruit export adapter ALYREF/THOC4 to intronless mRNA; its ATPase activity is cooperatively stimulated by RNA and ALYREF/THOC4 and ATP hydrolysis is thought to trigger the dissociation from RNA to allow the association of ALYREF/THOC4 and the NXF1-NXT1 heterodimer. Involved in transcription elongation and genome stability.
Cellular Location Nucleus. Nucleus speckle. Cytoplasm. Note=Can translocate to the cytoplasm in the presence of MX1. TREX complex assembly seems to occur in regions surrounding nuclear speckles known as perispeckles
Volume 50 µl
Citations (0)

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Background

Involved in nuclear export of spliced and unspliced mRNA. Assembling component of the TREX complex which is thought to couple mRNA transcription, processing and nuclear export, and specifically associates with spliced mRNA and not with unspliced pre-mRNA. TREX is recruited to spliced mRNAs by a transcription- independent mechanism, binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export to the cytoplasm via the TAP/NFX1 pathway. May undergo several rounds of ATP hydrolysis during assembly of TREX to drive subsequent loading of components such as ALYREF/THOC and CHTOP onto mRNA. The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production. Also associates with pre-mRNA independent of ALYREF/THOC4 and the THO complex. Involved in the nuclear export of intronless mRNA; the ATP-bound form is proposed to recruit export adapter ALYREF/THOC4 to intronless mRNA; its ATPase activity is cooperatively stimulated by RNA and ALYREF/THOC4 and ATP hydrolysis is thought to trigger the dissociation from RNA to allow the association of ALYREF/THOC4 and the NXF1-NXT1 heterodimer. Involved in transcription elongation and genome stability.

References

Peelman L.,et al.Genomics 26:210-218(1995).
Kalnine N.,et al.Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases.
Suzuki Y.,et al.Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases.
Shiina T.,et al.Genetics 173:1555-1570(2006).
Mungall A.J.,et al.Nature 425:805-811(2003).

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$ 395.00
Cat# ALS17258
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