|Application ||WB, IHC-P|
|Predicted||Human, Mouse, Rat|
|Calculated MW||40361 Da|
|Dilution||IHC-P (10 µg/ml), WB (0.5 - 1 µg/ml)|
|Other Names||GNAI1, Gi1 protein alpha subunit, Gi|
|Target/Specificity||Endogenous levels of mouse and rat G alpha(i). It is predicted to react with human G alpha(i) according to sequence homology. Positive Control: Mouse and Rat brain.|
|Reconstitution & Storage||Lyophilized from PBS, pH 7.4, 0.02% sodium azide. Store lyophilized at -20°C. The reconstituted product can be stored for short term at 4 °C or long term at -20 °C or below. Avoid freeze/thaw cycles.|
|Precautions||Anti-GNAI1 / Gi Antibody (N-Terminus) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The G(i) proteins are involved in hormonal regulation of adenylate cyclase: they inhibit the cyclase in response to beta-adrenergic stimuli. The inactive GDP-bound form prevents the association of RGS14 with centrosomes and is required for the translocation of RGS14 from the cytoplasm to the plasma membrane. May play a role in cell division.|
|Cellular Location||Nucleus. Cytoplasm. Cell membrane. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Note=Localized at the plasma membrane throughout mitosis. Colocalizes with RIC8A and RGS14 at the plasma membrane (By similarity). Localizes in the centrosomes of interphase and mitotic cells, but not in centrosomes during cytokinesis. Detected at the cleavage furrow or the midbody|
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