|Application ||WB, IHC-P, IHC-F, IP, Flo|
|Predicted||Human, Mouse, Rat, Rabbit, Hamster, Chicken, Rhesus, Sheep, Xenopus, Horse, Dog, Cat|
|Calculated MW||28769 Da|
|Dilution||Flo (1:100), IHC-Fr, IHC-P (10 µg/ml), IP, WB (1:1000)|
|Other Names||PCNA, Cyclin|
|Target/Specificity||Recognizes the proliferating cell nuclear antigen, also known as PCNA or cyclin. PCNA is a 261 amino acid ~28 kD nuclear protein vital for cellular DNA synthesis at the replication fork through its interaction with. PCNA is the auxilliary protein for ...|
|Reconstitution & Storage||Purified|
|Precautions||Anti-PCNA Antibody (clone PC10) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'- 5' exonuclease and 3'-phosphodiesterase, but not apurinic- apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways (PubMed:24939902). Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion.|
|Cellular Location||Nucleus. Note=Colocalizes with CREBBP, EP300 and POLD1 to sites of DNA damage (PubMed:24939902). Forms nuclear foci representing sites of ongoing DNA replication and vary in morphology and number during S phase. Together with APEX2, is redistributed in discrete nuclear foci in presence of oxidative DNA damaging agents.|
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