|Application ||WB, IHC-P, E|
|Calculated MW||47522 Da|
|Dilution||ELISA, IHC-P (5 µg/ml), WB (1:500 - 1:2000)|
|Other Names||CENPU, CENP-U, CENP50, CENPU50, Centromere protein U, ICEN24, KLIP1, Polo-box-interacting protein 1, MLF1-interacting protein, CENP-50, Centromere protein of 50 kDa, MLF1 interacting protein, MLF1IP, PBIP1|
|Target/Specificity||A BLAST analysis was used to suggest cross-reactivity with MLF1IP protein from human, chimpanzee (98%), horse (72%), bovine (69%), dog (65%), mouse (56%), rat (55%) and chicken (47%) sources based on homology with the immunizing sequence. Reactivity ...|
|Reconstitution & Storage||Immunoaffinity purified|
|Precautions||Anti-CENPU / MLF1IP Antibody (N-Terminus) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||ICEN24, KLIP1, MLF1IP, PBIP1|
|Function||Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. Plays an important role in the correct PLK1 localization to the mitotic kinetochores. A scaffold protein responsible for the initial recruitment and maintenance of the kinetochore PLK1 population until its degradation. Involved in transcriptional repression.|
|Cellular Location||Cytoplasm. Nucleus. Chromosome, centromere, kinetochore. Note=Localizes in the kinetochore domain of centromeres. Colocalizes with PLK1 at the interzone between the inner and the outer kinetochore plates|
|Tissue Location||Expressed at high levels in the testis, fetal liver, thymus, bone marrow and at lower levels in the lymph nodes, placenta, colon and spleen. Present in all cell lines examined, including B-cells, T-cells, epithelial cells and fibroblast cells Expressed at high levels in glioblastoma cell lines|
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