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> home > Products > Primary Antibodies > Antibody Collections > GPCR Antibodies > Anti-TRIM25 Antibody (clone 5C3)
Anti-TRIM25 Antibody (clone 5C3)
Mouse Anti Human Monoclonal Antibody
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- CITATIONS
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- BACKGROUND
Application 
| WB, IHC-P, IF, E |
|---|---|
| Primary Accession | Q14258 |
| Predicted | Human |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1,k |
| Clone Names | 5C3 |
| Calculated MW | 70973 Da |
| Dilution | ELISA, IF (20 µg/ml), IHC-P (3 - 10 µg/ml), WB |
| Gene ID | 7706 |
|---|---|
| Alias Symbol | TRIM25 |
| Other Names | TRIM25, EFP, Zinc finger protein 147, Z147, Zinc finger protein-147, ZNF147, Tripartite motif containing 25, RING finger protein 147, RNF147, Tripartite motif-containing 25 |
| Target/Specificity | Human TRIM25 |
| Reconstitution & Storage | Protein A purified |
| Precautions | Anti-TRIM25 Antibody (clone 5C3) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | TRIM25 |
|---|---|
| Synonyms | EFP {ECO:0000303|PubMed:8248217}, RNF147 |
| Function | Functions as a ubiquitin E3 ligase and as an ISG15 E3 ligase (PubMed:16352599). Involved in innate immune defense against viruses by mediating ubiquitination of RIGI and IFIH1 (PubMed:17392790, PubMed:30193849, PubMed:33849980, PubMed:29357390, PubMed:31710640, PubMed:36045682). Mediates 'Lys-63'-linked polyubiquitination of the RIGI N-terminal CARD-like region and may play a role in signal transduction that leads to the production of interferons in response to viral infection (PubMed:17392790, PubMed:23950712). Mediates 'Lys-63'- linked polyubiquitination of IFIH1 (PubMed:30193849). Promotes ISGylation of 14-3-3 sigma (SFN), an adapter protein implicated in the regulation of a large spectrum signaling pathway (PubMed:16352599, PubMed:17069755). Mediates estrogen action in various target organs (PubMed:22452784). Mediates the ubiquitination and subsequent proteasomal degradation of ZFHX3 (PubMed:22452784). Plays a role in promoting the restart of stalled replication forks via interaction with the KHDC3L-OOEP scaffold and subsequent ubiquitination of BLM, resulting in the recruitment and retainment of BLM at DNA replication forks (By similarity). Plays an essential role in the antiviral activity of ZAP/ZC3HAV1; an antiviral protein which inhibits the replication of certain viruses. Mechanistically, mediates 'Lys-63'- linked polyubiquitination of ZAP/ZC3HAV1 that is required for its optimal binding to target mRNA (PubMed:28202764, PubMed:28060952). Mediates also the ubiquitination of various substrates implicated in stress granule formation, nonsense-mediated mRNA decay, nucleoside synthesis and mRNA translation and stability (PubMed:36067236). |
| Cellular Location | Cytoplasm. Cytoplasm, Stress granule. Nucleus {ECO:0000250|UniProtKB:Q61510} |
| Tissue Location | Expressed in breast tumors (at protein level). Ubiquitous. |
Research Areas
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