|Application ||WB, IHC-P, IF, E|
|Calculated MW||70973 Da|
|Dilution||ELISA, IF (20 µg/ml), IHC-P (3 - 10 µg/ml), WB|
|Other Names||TRIM25, EFP, Zinc finger protein 147, Z147, Zinc finger protein-147, ZNF147, Tripartite motif containing 25, RING finger protein 147, RNF147, Tripartite motif-containing 25|
|Reconstitution & Storage||Protein A purified|
|Precautions||Anti-TRIM25 Antibody (clone 5C3) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||EFP, RNF147, ZNF147|
|Function||Functions as a ubiquitin E3 ligase and as an ISG15 E3 ligase. Involved in innate immune defense against viruses by mediating ubiquitination of DDX58. Mediates 'Lys-63'-linked polyubiquitination of the DDX58 N-terminal CARD-like region which is crucial for triggering the cytosolic signal transduction that leads to the production of interferons in response to viral infection. Promotes ISGylation of 14-3-3 sigma (SFN), an adapter protein implicated in the regulation of a large spectrum signaling pathway. Mediates estrogen action in various target organs. Mediates the ubiquitination and subsequent proteasomal degradation of ZFHX3 (PubMed:22452784).|
|Cellular Location||Cytoplasm. Note=Colocalized with DDX58 at cytoplasmic perinuclear bodies|
|Tissue Location||Expressed in breast tumors (at protein level). Ubiquitous.|
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