|Application ||WB, IHC-P|
|Predicted||Human, Mouse, Rat|
|Calculated MW||87680 Da|
|Dilution||IHC-P (1:100), WB (1:500 - 1:2000)|
|Other Names||CUL4A, CUL-4A, Cullin-4A, Cullin 4A|
|Target/Specificity||Human Cullin 4A / CUL4A|
|Reconstitution & Storage||Affinity purified|
|Precautions||Anti-Cullin 4A / CUL4A Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Core component of multiple cullin-RING-based E3 ubiquitin-protein ligase complexes which mediate the ubiquitination of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition component. DCX(DET1-COP1) directs ubiquitination of JUN. DCX(DDB2) directs ubiquitination of XPC. DCX(DDB2) ubiquitinates histones H3-H4 and is required for efficient histone deposition during replication-coupled (H3.1) and replication-independent (H3.3) nucleosome assembly, probably by facilitating the transfer of H3 from ASF1A/ASF1B to other chaperones involved in histone deposition. DCX(DTL) plays a role in PCNA-dependent polyubiquitination of CDT1 and MDM2-dependent ubiquitination of TP53 in response to radiation-induced DNA damage and during DNA replication. In association with DDB1 and SKP2 probably is involved in ubiquitination of CDKN1B/p27kip. Is involved in ubiquitination of HOXA9. DCX(DTL) directs autoubiquitination of DTL.|
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