Anti-NUCB1 / Nucleobindin Antibody (clone 1H8-2C11)

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18,000+ products cover the ten top research categories, including cancer, metabolism, cardiovascular, neuroscience, and stem cells. Each antibody is crafted with care according to rigorous protocols for immunogen design and preparation, presentation to host animal, and high-affinity purification against the antigen. Quality is embedded in the production process to assure the best antibody for you.

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Crown Products

Crown antibodies pass additional stringent quality requirements, including extended control sets, uniform results against multiple biologically relevant cell lines and tissues, and function in multiple applications.

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New Products

Abgent develops 1000+ new products per year. We provide tools for path-breaking research by developing antibodies that detect a comprehensive library of novel and established targets. For established targets we seek to add antibodies that recognize new epitopes, including post-translational modifications such as phosphorylation and methylation. For new targets we consult with leading experts to accelerate development of antibodies that will propel state-of-the-art research in cellular health and disease.

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Current Promotion

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Current Promotion

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Research Focus

Greneral Collections

Peptides

SARS Peptides

Individual peptides for SARS-CoV-2 Spike glycoprotein. In order to study the specificity of cellular immune responses against SARS CoV-2 and potential immunity caused by other human Corona Viruses, Abcepta provides Spike peptide individually, as pools and in plate. These peptides can be used for antigen specific T-cell stimulation in T-cell assays or T-cell expansion.

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Synthetic Peptides

All peptides are manufactured in the San Diego, California. Custom peptide services include long peptides (>100 aa), cyclic peptides, difficult sequences, fluorescent labels, phospho-peptides and other post-translational modifications.

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Blocking Peptides

The majority of Abcepta antibodies are produced using peptide immunogens. These immuno-specific peptides can be used as blocking agents when using the complementary antibodies in a range of applications.

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Cell/Tissues/Lysates

Abcepta's portfolio of cell lines, tissues and lysates are drawn from a range of species and immortalized cell lines. All our cell lines and lysates are validated in key applications.

These tissue lysates can be used in applications such as SDS-PAGE and Western blotting. Whole cell lysates can be used as positive controls for applications such as ELISAs, immunoprecipitation (IP) and Western blotting. Obtaining high-quality whole cell lysates can be tedious as well as time consuming. Abcepta offers a comprehensive portfolio of whole cell lysates for research use. Browse our catalog to find the right whole cell lysate for your experiment.

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Citations

Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.

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Submit your citation using an Abcepta antibody to
info@abcepta.com, and receive a free "I Love Antibodies" mug.

Application Applications Legend: WB=Western Blot IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin-embedded Sections) IHC-F=Immunohistochemistry (Frozen Sections) IF=Immunofluorescence FC=Flow Cytopmetry IC=Immunochemistry ICC=Immunocytochemistry E=ELISA IP=Immunoprecipitation DB=Dot Blot CHIP=Chromatin Immunoprecipitation FA=Fluorescence Assay IEM=Immunoelectronmicroscopy EIA=Enzyme Immunoassay	WB, IHC-P, IF, E
Primary Accession	Q02818
Predicted	Human
Host	Mouse
Clonality	Monoclonal
Isotype	IgG1,k
Clone Names	1H8-2C11
Calculated MW	53879 Da
Dilution	ELISA, IF (10 µg/ml), IHC-P (3 - 10 µg/ml), WB

Gene ID	4924
Alias Symbol	NUCB1
Other Names	NUCB1, CALNUC, Nucleobindin-1, Nucleobindin 1, NUC, Nucleobindin
Target/Specificity	Human NUCB1
Reconstitution & Storage	Protein A purified
Precautions	Anti-NUCB1 / Nucleobindin Antibody (clone 1H8-2C11) is for research use only and not for use in diagnostic or therapeutic procedures.

Name	NUCB1
Synonyms	NUC
Function	Major calcium-binding protein of the Golgi which may have a role in calcium homeostasis (By similarity). Acts as a non-receptor guanine nucleotide exchange factor which binds to and activates alpha subunits of guanine nucleotide-binding proteins (G proteins) (By similarity).
Cellular Location	Golgi apparatus, cis-Golgi network membrane {ECO:0000250\|UniProtKB:Q63083}; Peripheral membrane protein {ECO:0000250\|UniProtKB:Q63083}; Lumenal side {ECO:0000250\|UniProtKB:Q63083}. Cytoplasm {ECO:0000250\|UniProtKB:Q63083}. Secreted {ECO:0000250\|UniProtKB:Q63083}. Note=A small fraction of the protein may be cytoplasmic. {ECO:0000250\|UniProtKB:Q63083}
Tissue Location	Expressed both in fetal and adult heart, lung, liver, kidney and brain, and in adult skeletal muscle, placenta and pancreas

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