|Application ||WB, IHC-P, E|
|Calculated MW||67769 Da|
|Dilution||ELISA, IHC-P (1:50), WB|
|Other Names||NEIL3, DNA glycosylase/AP lyase Neil3, FGP2, DNA glycosylase FPG2, DNA glycosylase hFPG2, FPG2, HFPG2, Endonuclease 8-like 3, Endonuclease VIII-like 3, HNEI3, Nei-like protein 3, NEI3|
|Target/Specificity||NEIL3 Antibody detects endogenous levels of total NEIL3 protein.|
|Reconstitution & Storage||Immunoaffinity purified|
|Precautions||Anti-NEI3 / NEIL3 Antibody (aa491-540) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||DNA glycosylase which prefers single-stranded DNA (ssDNA), or partially ssDNA structures such as bubble and fork structures, to double-stranded DNA (dsDNA). In vitro, displays strong glycosylase activity towards the hydantoin lesions spiroiminodihydantoin (Sp) and guanidinohydantoin (Gh) in both ssDNA and dsDNA; also recognizes FapyA, FapyG, 5-OHU, 5-OHC, 5- OHMH, Tg and 8-oxoA lesions in ssDNA. No activity on 8-oxoG detected. Also shows weak DNA-(apurinic or apyrimidinic site) lyase activity. In vivo, appears to be the primary enzyme involved in removing Sp and Gh from ssDNA in neonatal tissues. Seems to be an important facilitator of cell proliferation in certain populations, for example neural stem/progenitor cells and tumor cells, suggesting a role in replication-associated DNA repair.|
|Tissue Location||Expressed in keratinocytes and embryonic fibroblasts (at protein level). Also detected in thymus, testis and fetal lung primary fibroblasts.|
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