|Application ||WB, IHC-P, IF, ICC|
|Calculated MW||19330 Da|
|Dilution||ICC (1:100 - 1:500), IF, IHC-P (1:100), WB (1:500 - 1:1000)|
|Other Names||SRSF3, SRp20, Pre-mRNA-splicing factor SRP20, SFRS3|
|Target/Specificity||Recognizes endogenous levels of SRSF3 protein.|
|Reconstitution & Storage||Immunoaffinity purified|
|Precautions||Anti-SRSF3 / SRP20 Antibody (C-Terminus) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Splicing factor that specifically promotes exon- inclusion during alternative splicing (PubMed:26876937). Interaction with YTHDC1, a RNA-binding protein that recognizes and binds N6-methyladenosine (m6A)-containing RNAs, promotes recruitment of SRSF3 to its mRNA-binding elements adjacent to m6A sites, leading to exon-inclusion during alternative splicing (PubMed:26876937). Also functions as export adapter involved in mRNA nuclear export (PubMed:11336712, PubMed:18364396, PubMed:28984244). Binds mRNA which is thought to be transferred to the NXF1-NXT1 heterodimer for export (TAP/NXF1 pathway); enhances NXF1-NXT1 RNA-binding activity (PubMed:11336712, PubMed:18364396). Involved in nuclear export of m6A-containing mRNAs via interaction with YTHDC1: interaction with YTHDC1 facilitates m6A-containing mRNA-binding to both SRSF3 and NXF1, promoting mRNA nuclear export (PubMed:28984244). RNA-binding is semi-sequence specific (PubMed:17036044).|
|Cellular Location||Nucleus. Nucleus speckle. Cytoplasm. Note=Recruited to nuclear speckles following interaction with YTHDC1.|
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