|Application ||WB, IHC-P, E|
|Calculated MW||18502 Da|
|Dilution||ELISA (0.05 - 0.2 µg/ml), IHC-P (5 µg/ml), WB (0.5 - 1 µg/ml)|
|Other Names||CFL1, 18 kDa phosphoprotein, Cofilin, CFL, Cofilin 1 (non-muscle), Cofilin, non-muscle isoform, p18|
|Target/Specificity||Endogenous levels of human and mouse cofilin protein. It is predicted to react with cynomolgus monkey, sheep, pig, and rat cofilin protein according to sequence homology.|
|Reconstitution & Storage||Immunoaffinity purified|
|Precautions||Anti-CFL1 / Cofilin Antibody (N-Terminus) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Binds to F-actin and exhibits pH-sensitive F-actin depolymerizing activity. Regulates actin cytoskeleton dynamics. Important for normal progress through mitosis and normal cytokinesis. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required for the up-regulation of atypical chemokine receptor ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation (PubMed:11812157, PubMed:15580268, PubMed:21834987, PubMed:23633677). Required for neural tube morphogenesis and neural crest cell migration (By similarity).|
|Cellular Location||Nucleus matrix. Cytoplasm, cytoskeleton. Cell projection, ruffle membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection, lamellipodium membrane; Peripheral membrane protein; Cytoplasmic side. Note=Colocalizes with the actin cytoskeleton in membrane ruffles and lamellipodia. Detected at the cleavage furrow and contractile ring during cytokinesis. Almost completely in nucleus in cells exposed to heat shock or 10% dimethyl sulfoxide|
|Tissue Location||Widely distributed in various tissues.|
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