|Application ||WB, IHC-P|
|Calculated MW||32308 Da|
|Dilution||IHC-P (1:100), WB (1:500 - 1:1000)|
|Other Names||MARCH1, MARCH-I, RING finger protein 171, RNF171|
|Target/Specificity||Recognizes endogenous levels of 42064 protein.|
|Reconstitution & Storage||Immunoaffinity purified|
|Precautions||Anti-MARCH1 Antibody (Internal) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||E3 ubiquitin-protein ligase that mediates ubiquitination of TFRC, CD86, FAS and MHC class II proteins, such as HLA-DR alpha and beta, and promotes their subsequent endocytosis and sorting to lysosomes via multivesicular bodies. By constitutively ubiquitinating MHC class II proteins in immature dendritic cells, down-regulates their cell surface localization thus sequestering them in the intracellular endosomal system.|
|Cellular Location||Golgi apparatus, trans-Golgi network membrane; Multi-pass membrane protein Lysosome membrane; Multi-pass membrane protein. Cytoplasmic vesicle membrane; Multi-pass membrane protein. Late endosome membrane; Multi-pass membrane protein Early endosome membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein|
|Tissue Location||Expressed in antigen presenting cells, APCs, located in lymph nodes and spleen. Also expressed in lung Expression is high in follicular B-cells, moderate in dendritic cells and low in splenic T-cells.|
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