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GST AntibodyPurified Mouse Monoclonal Antibody (Mab)
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AM1011b | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
- Reviews
- Protocols
- Backgrounds
GST Antibody - Product info | |
| Application | WB
|
| Concentration | 0.25 mg/ml |
| Isotype | IgG1 k |
| Clone Names | 21CT54.13.1 / 21CT54.13.7 |
| Calculated MW | 26000 Da |
GST Antibody - Additional info | |
| Target/Specificity Purified recombinant GST fusion protein was used to produced this monoclonal antibody. | |
| Dilution WB~~1:100-500 | |
| Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions GST Antibody is for research use only and not for use in diagnostic or therapeutic procedures. | |
GST Antibody - Application data
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Western blot analysis of anti-GST Mab in recombinant GST protein. GST(arrow) was detected using the purified Mab (1:60 dilution).
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Western blot analysis of anti-GST Mab in recombinant GST protein. GST(arrow) was detected using the purified Mab (1:60 dilution).
GST Antibody - Research Areas
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BACKGROUND
Glutathione S-transferase (GST) was originally cloned from parasite Schistosoma japonicum and it is now a widely used protein fusion partner. Vectors containing GST Tag have been developed for both prokaryotic and eukaryotic systems. The GST fusion proteins are easily purified from cell lysates by affinity chromatography using Glutathione Sepharose 4B to elute out the GST fusion protein from the column with a denaturing form of glutathione. Using the Abgent anti-GST antibody provides a simple solution to detect the expression of GST fusion proteins in cells.
REFERENCES
Smith, D.B. and Johnson, K.S., (1988). Gene 67, 31. Parker, M.W. et al., (1990) J. Mol. Biol. 213, 221. Toye, B. et al., (1990) Infect. Immun. 58, 3909. Guan, K.L. and Dixon, J.E. (1991) Anal. Biochem. 192, 262
