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ATG12 AntibodyPurified Mouse Monoclonal Antibody (Mab)
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AM1816a | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
- Reviews
- Protocols
- Backgrounds
ATG12 Antibody - Product info | |
| Application | WB, IHC, IF
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| Primary Accession | O94817 |
| Reactivity | Human |
| Concentration | 0.25 mg/ml |
| Isotype | IgG2bκ |
| Clone Names | 43CT73.3.5.5.4 |
| Calculated MW | 15113 Da |
ATG12 Antibody - Additional info | |
| Gene ID 9140 | |
| Other Names ATG12; APG12; APG12L; Ubiquitin-like protein ATG12; Autophagy-related protein 12 | |
| Target/Specificity APG12L recombinant protein is used to produce this monoclonal antibody. | |
| Dilution WB~~1:200~2000 IHC~~1:50~200 IF~~1:10~50 | |
| Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions ATG12 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. | |
ATG12 Antibody - Protein Information | |
| Name ATG12 | |
| Synonyms APG12, APG12L | |
| Function Ubiquitin-like protein required for autophagy. Conjugated to ATG3 and ATG5 | |
| Cellular Location Cytoplasm (By similarity). | |
| Tissue Location Ubiquitous. | |
ATG12 Antibody - Related products
AP1151a: ATG12 Antibody (N-term)
AP1816a: ATG12 Antibody (N-term)
RI10451: ATG12 predesign siRNA
DC03063: Human ATG12 cDNA Clone
BP1151a: ATG12 Antibody (N-term) Blocking Peptide
BP1151b: Phospho-Histone H3-S10 Antibody Blocking Peptide
BP1151c: Phospho-Histone H3-S10 Antibody Blocking Peptide
ATG12 Antibody - Application data
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Western blot analysis of anti-APG12 Monoclonal Antibody (Cat.#AM1816a) by APG12 recombinant protein. APG12(arrow) was detected using the purified Mab.
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Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue and lung carcinoma tissue reacted with APG12L Monoclonal Antibody (Cat.#AM1816a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with APG12L Monoclonal Antibody (Cat.#AM1816a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Atg12 antibody tested in HEK cells. Fixed in PFA permeablilized with 0.2% Saponin. Blocked and antibodies in 10% goat serum. Secondary was mouse 555. The right is in full medium (FM) and the left with EBSS (no leupeptin)(Provided by Nicole McKnight & Sharon Tooze).
ATG12 Antibody - Research Areas
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BACKGROUND
Autophagy is a process of bulk protein degradation in which cytoplasmic components, including organelles, are enclosed in double-membrane structures called autophagosomes and delivered to lysosomes or vacuoles for degradation. ATG12 is the human homolog of a yeast protein involved in autophagy (Mizushima et al., 1998 [PubMed 9852036]).
REFERENCES
Control of basal autophagy by calpain1 mediated cleavage of ATG5. Xia HG, et al. Autophagy, 2010 Jan. PMID 19901552. Frameshift mutations of autophagy-related genes ATG2B, ATG5, ATG9B and ATG12 in gastric and colorectal cancers with microsatellite instability. Kang MR, et al. J Pathol, 2009 Apr. PMID 19197948. Regulation of autophagy by the p300 acetyltransferase. Lee IH, et al. J Biol Chem, 2009 Mar 6. PMID 19124466. Mitochondrial DNA deletions and chloramphenicol treatment stimulate the autophagic transcript ATG12. Prigione A, et al. Autophagy, 2007 Jul-Aug. PMID 17457038. Large-scale mapping of human protein-protein interactions by mass spectrometry. Ewing RM, et al. Mol Syst Biol, 2007. PMID 17353931.
