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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   BRAF Antibody   

BRAF Antibody

Purified Mouse Monoclonal Antibody (Mab)

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  • WB - BRAF Antibody AM1821a
    All lanes : Anti-BRAF Antibody at 1:1000 dilution Lane 1: MCF-7 whole cell lysate Lane 2: K562 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 84 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • IF - BRAF Antibody AM1821a
    Fluorescent image of C2C12 cell stained with BRAF Antibody(Cat#AM1821a/SG100521B).C2C12 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with BRAF primary antibody (1:25, 1 h at 37℃. For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-mouse antibody (green) was used (1:400, 50 min at 37℃.Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃.BRAF immunoreactivity is localized to Cytoplasm significantly.
  • WB - BRAF Antibody AM1821a
    Western blot analysis of anti-BRAF Antibody in HL-60 cell line lysates (35μg/lane). BRAF (arrow) was detected using the purified Mab.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P15056
Other Accession NP_004324
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG1κ
Clone Names 125CT13.3.5
Calculated MW 84437 Da
Additional Information
Gene ID 673
Other Names Serine/threonine-protein kinase B-raf, Proto-oncogene B-Raf, p94, v-Raf murine sarcoma viral oncogene homolog B1, BRAF, BRAF1, RAFB1
Target/Specificity This BRAF Monoclonal antibody is generated from mouse immunized with BRAF recombinant protein.
Dilution WB~~1:500~16000
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsBRAF Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Synonyms BRAF1, RAFB1
Function Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron. Phosphorylates MAP2K1, and thereby contributes to the MAP kinase signal transduction pathway.
Cellular Location Nucleus. Cytoplasm. Cell membrane. Note=Colocalizes with RGS14 and RAF1 in both the cytoplasm and membranes.
Tissue Location Brain and testis.
Research Areas
Citations (0)

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This gene encodes a protein belonging to the raf/mil family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. Mutations in this gene have also been associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung. A pseudogene, which is located on chromosome X, has been identified for this gene.


Mutational analyses of the BRAF, KRAS, and PIK3CA genes in oral squamous cell carcinoma. Bruckman KC, et al. Oral Surg Oral Med Oral Pathol Oral Radiol Endod, 2010 Aug 31. PMID 20813562.
BRAF Mutation Is Rare in Advanced-Stage Low-Grade Ovarian Serous Carcinomas. Wong KK, et al. Am J Pathol, 2010 Aug 27. PMID 20802181.
NRAS mutations are rare in colorectal cancer. Irahara N, et al. Diagn Mol Pathol, 2010 Sep. PMID 20736745.
Molecular Testing for Somatic Mutations Improves the Accuracy of Thyroid Fine-needle Aspiration Biopsy. Moses W, et al. World J Surg, 2010 Aug 12. PMID 20703476.
Identification of several novel non-p.R132 IDH1 variants in thyroid carcinomas. Hemerly JP, et al. Eur J Endocrinol, 2010 Aug 11. PMID 20702649.

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$ 295.00
$ 99.00
Cat# AM1821a
(40 western blots)
Availability: In Stock
Bulk Size
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