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Beta-Actin Antibody

Mouse Monoclonal Antibody (Mab)

Country
United States
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Ordering Information
Catalog # SizeAvailability Price  
AM1829B 400 µl
(40 western blots)
In Stock $ 265.00 Add to cart
AM1829B-ev 80 µl
(8 western blots)
In Stock $ 95.00 Add to cart
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  • Specification
  • Citations : 5
  • Reviews
  • Protocols
  • Background

Beta-Actin Antibody - Product Information

Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • ICC=Immunocytochemistry
  • CHIP=Chromatin Immunoprecipitation
  • ELISA=ELISA
  • IHCF=Immunohistochemistry (Frozen Sections)
  • FA=Fluorescence Assay
  • IHCP=Immunohistochemistry (Paraffin-embedded Sections)
  • IC=Immunochemistry
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
  • IH=Immunohistology (Frozen & formalin-fixed)
  • EM=Electronmicroscopy
IHC, IF, WB
Primary AccessionP60709
Other AccessionA2BDB0, P63259, P63260, P63261, Q5ZMQ2, P63258, P60711, Q6QAQ1, P60710, Q4R561, P60706, P60712, P53505, P60708, P60713
ReactivityHuman, Mouse
PredictedXenopus, Bovine, Chicken, Monkey, Pig, Rat
HostMouse
ClonalityMonoclonal
Isotype IgG1,Igk
Clone Names137CT26.1.1

Beta-Actin Antibody - Additional Information

Gene ID 60
Other Names
Actin, cytoplasmic 1, Beta-actin, Actin, cytoplasmic 1, N-terminally processed, ACTB
Target/Specificity
This ACTB Monoclonal antibody is generated from mouse immunized with ACTB recombinant protein.
Dilution
IHC~~1:10~50
IF~~1:10~50
WB~~1:1000
Format
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
Storage
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions
Beta-Actin Antibody is for research use only and not for use in diagnostic or therapeutic procedures.

Beta-Actin Antibody - Protein Information

Name ACTB
Function
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Cellular Location
Cytoplasm, cytoskeleton. Note=Localized in cytoplasmic mRNP granules containing untranslated mRNAs

Beta-Actin Antibody - Related Products

AM1021a: Beta-actin Antibody (Ascites)

AM1021B: Beta-actin Antibody

AM1829a: ACTB Antibody (Ascites)

AO1215a: beta-Actin Antibody

AP50810: Anti-beta-Actin antibody

AP50879: Anti-beta-Actin (Loading Control) antibody

Beta-Actin Antibody - Research Areas

Application Data

  • IHC - Beta-Actin  Antibody AM1829B

    Immunohistochemical analysis of paraffin-embedded H.spleen section using Beta-Actin Antibody(Cat#AM1829b). AM1829b was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

  • IHC - Beta-Actin  Antibody AM1829B

    ACTB Antibody (AM1829b)immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ACTB Antibody for immunohistochemistry. Clinical relevance has not been evaluated.

  • IF - Beta-Actin  Antibody AM1829B

    Confocal immunofluorescent analysis of ACTB Antibody (Cat#AM1829b) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green). DAPI was used to stain the cell nuclear (blue).

  • WB - Beta-Actin  Antibody AM1829B

    Western blot analysis of anti-ACTB Antibody (Cat. #AM1829b) in K562, HL-60,Hela cell line, mouse spleen, mouse liver tissue lysates, mouse NIH-3T3 cell line lysate and mouse cerebellum, mouse brain tissue lysates (35ug/lane). ACTB (arrow) was detected using the purified Mab.

  • IHC - Beta-Actin  Antibody AM1829B
  • IHC - Beta-Actin  Antibody AM1829B
  • IF - Beta-Actin  Antibody AM1829B
  • WB - Beta-Actin  Antibody AM1829B

ELITE™ Custom Antibody Services

  • Abnormal Accumulation of Desmin in Gastrocnemius Myofibers of Patients with Peripheral Artery Disease: Associations with Altered Myofiber Morphology and Density, Mitochondrial Dysfunction and Impaired Limb Function. Author : Koutakis P1, Miserlis D1, Myers SA1, Kim JK1, Zhu Z1, Papoutsi E1, Swanson SA1, Haynatzki G1, Ha DM1, Carpenter LA1, McComb RD1, Johan
    J Histochem Cytochem. 2015 Jan 9. pii: 0022155415569348. [Epub ahead of print]
    PubMed® Id : 25575565
  • Androgen receptor silences thioredoxin-interacting protein and competitively inhibits glucocorticoid receptor-mediated apoptosis in pancreatic â-cells. Author : Harada N1, Katsuki T, Takahashi Y, Masuda T, Yoshinaga M, Adachi T, Izawa T, Kuwamura M, Nakano Y, Yamaji R, Inui H.
    J Cell Biochem. 2015 Jan 5. doi: 10.1002/jcb.25054. [Epub ahead of print]
    PubMed® Id : 25639671
  • Functional analyses of the three simian hemorrhagic Fever virus nonstructural protein 1 papain-like proteases. Author : Vatter HA1, Di H1, Donaldson EF2, Radu GU1, Maines TR1, Brinton MA3.
    J Virol. 2014 Aug 15;88(16):9129-40. doi: 10.1128/JVI.01020-14. Epub 2014 Jun 4.
    PubMed® Id : 24899184
  • Addiction to multiple oncogenes can be exploited to prevent the emergence of therapeutic resistance. Author : Choi PS1, Li Y1, Felsher DW2.
    Proc Natl Acad Sci U S A. 2014 Aug 12;111(32):E3316-24. doi: 10.1073/pnas.1406123111. Epub 2014 Jul 28.
    PubMed® Id : 25071175
  • Evidence for a novel antioxidant function and isoform-specific regulation of the human p66Shc gene. Author : Miyazawa M1, Tsuji Y2.
    Mol Biol Cell. 2014 Jul 1;25(13):2116-27. doi: 10.1091/mbc.E13-11-0666. Epub 2014 May 7.
    PubMed® Id : 24807908

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BACKGROUND

This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins.

REFERENCES

Sex-specific proteome differences in the anterior cingulate cortex of schizophrenia. Martins-de-Souza D, et al. J Psychiatr Res, 2010 Apr 8. PMID 20381070. Identification of a hormone-regulated dynamic nuclear actin network associated with estrogen receptor alpha in human breast cancer cell nuclei. Ambrosino C, et al. Mol Cell Proteomics, 2010 Jun. PMID 20308691. Contribution of rearranged actin structures to the spread of Ectromelia virus infection in vitro. Boratynska A, et al. Acta Virol, 2010. PMID 20201613. Molecular mechanisms underlying nucleocytoplasmic shuttling of actinin-4. Kumeta M, et al. J Cell Sci, 2010 Apr 1. PMID 20197409. Tyrosine phosphorylation of cofilin at Y68 by v-Src leads to its degradation through ubiquitin-proteasome pathway. Yoo Y, et al. Oncogene, 2010 Jan 14. PMID 19802004.