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ALDH1A1 AntibodyPurified Mouse Monoclonal Antibody (Mab)
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AM1846b | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
| AM1846b-ev20 | 20 ug 100 ul | In Stock | $ 95.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
- Reviews
- Protocols
- Backgrounds
ALDH1A1 Antibody - Product info | |
| Application | IF, WB, IHC
|
| Primary Accession | P00352 |
| Other Accession | NP_000680.2 |
| Reactivity | Human |
| Concentration | 0.25 mg/ml |
| Isotype | Mouse IgG1 |
| Clone Names | 152CT1.2.2 |
| Calculated MW | 54862 Da |
ALDH1A1 Antibody - Additional info | |
| Gene ID 216 | |
| Other Names ALDH1A1; ALDC; ALDH1; PUMB1; Retinal dehydrogenase 1; ALDH-E1; ALHDII; Aldehyde dehydrogenase family 1 member A1; Aldehyde dehydrogenase, cytosolic | |
| Target/Specificity This ALDH1A1 monoclonal antibody is generated from mouse immunized with ALDH1A1 recombinant protein. | |
| Dilution IF~~1:10~50 WB~~1:500~1000 IHC~~1:50~100 | |
| Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions ALDH1A1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. | |
ALDH1A1 Antibody - Protein Information | |
| Name ALDH1A1 | |
| Synonyms ALDC, ALDH1, PUMB1 | |
| Function Binds free retinal and cellular retinol-binding protein- bound retinal. Can convert/oxidize retinaldehyde to retinoic acid (By similarity) | |
| Cellular Location Cytoplasm. | |
ALDH1A1 Antibody - Related products
AM1846a: ALDH1A1 Antibody (Ascites)
AP1445a: ALDH1A1 Antibody (N-term)
AP1465c: ALDH1A1 Antibody (Center)
RI10225: ALDH1A1 predesign siRNA
LY12320a: AL1A1 Over-expression Lysate
BP1445a: ALDH1A1 Antibody (N-term) Blocking Peptide
BP1465c: ALDH1A1 Antibody (Center) Blocking Peptide
ALDH1A1 Antibody - Application data
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Fluorescent confocal image of HepG2 cells stained with ALDH1A1 antibody. HepG2 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AM1846b ALDH1A1 primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-mouse antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). ALDH1A1 immunoreactivity is localized to the cytoplasm of HepG2 cells.
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ALDH1A1 Monoclonal Antibody(Cat. #AM1846b) western blot analysis in NCI-H460 cell line lysates (15ug/lane).This demonstrates the ALDH1A1 antibody detected the ALDH1A1 protein (arrow).
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ALDH1A1 Monoclonal Antibody (Cat. #AM1846b) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ALDH1A1 Monoclonal Antibody for immunohistochemistry. Clinical relevance has not been evaluated.
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Confocal immunofluorescent analysis of ALDH1A1 Antibody (Cat#AM1846b) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green). DAPI was used to stain the cell nuclear (blue).
ALDH1A1 Antibody - Research Areas
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BACKGROUND
ALDH1A1 encodes a transcriptional regulator belonging to the SCY1-like family of kinase-like proteins. The protein has a divergent N-terminal kinase domain that is thought to be catalytically inactive, and can bind specific DNA sequences through its C-terminal domain. It activates transcription of the telomerase reverse transcriptase and DNA polymerase beta genes. The protein has been localized to the nucleus, and also to the cytoplasm and centrosomes during mitosis.
REFERENCES
References for protein: 1.Gong, Y., et al. Oncogene 28(12):1549-1560(2009) 2.Burman, J.L., et al. J. Biol. Chem. 283(33):22774-22786(2008) 3.Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007) References for HepG2 cell line: 1. Knowles BB, et al. (1980). Human hepatocellular carcinoma cell lines secrete the major plasma proteins and hepatitis B surface antigen. Science 209: 497-499.[ PubMed: 6248960]. 2. Darlington GJ, et al. (1987). Growth and hepatospecific gene expression of human hepatoma cells in a defined medium. In Vitro Cell. Dev. Biol. 23: 349-354.[PubMed: 3034851]. 3. Ihrke, G; Neufeld, EB; Meads, T; Shanks, MR; Cassio, D; Laurent, M; Schroer, TA; Pagano, RE et al. (1993). "WIF-B cells: an in vitro model for studies of hepatocyte polarity". Journal of Cell Biology 123 (6): 1761–1775. [PubMed:7506266]. 4. Mersch-Sundermann, V.; Knasmüller, S.; Wu, X. J.; Darroudi, F.; Kassie, F. (2004). "Use of a human-derived liver cell line for the detection of cytoprotective, antigenotoxic and cogenotoxic agents". Toxicology 198 (1–3): 329–340. [PubMed:15138059].
