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>   home   >   Products   >   Primary Antibodies   >   EIF4E Antibody (Ascites)   

EIF4E Antibody (Ascites)

Mouse Monoclonal Antibody (Mab)

     
  • WB - EIF4E Antibody (Ascites) AM1852a
    EIF4E Antibody (Cat. #AM1852a) western blot analysis in Jurkat cell line lysates (15μg/lane).This demonstrates the EIF4E antibody detected the EIF4E protein (arrow).
  • WB - EIF4E Antibody (Ascites) AM1852a
    EIF4E Antibody (Cat. #AM1852a) western blot analysis in mouse NIH-3T3 cell line lysates (15μg/lane).This demonstrates the EIF4E antibody detected the EIF4E protein (arrow).
  • FC - EIF4E Antibody (Ascites) AM1852a
    EIF4E Antibody (Cat. #AM1852a) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-mouse secondary antibodies were used for the analysis.
  • SPECIFICATION
  • CITATIONS: 4
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, FC, E
Primary Accession P06730
Other Accession NP_001959.1
Reactivity Human, Mouse
Host Mouse
Clonality Monoclonal
Isotype IgG1,K
Clone Names 163CT48.1.9
Calculated MW 25097 Da
Additional Information
Gene ID 1977
Other Names Eukaryotic translation initiation factor 4E, eIF-4E, eIF4E, eIF-4F 25 kDa subunit, mRNA cap-binding protein, EIF4E, EIF4EL1, EIF4F
Target/Specificity This EIF4E monoclonal antibody is generated from mouse immunized with EIF4E recombinant protein.
Dilution WB~~1:1000
FC~~1:10~50
Format Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsEIF4E Antibody (Ascites) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name EIF4E
Synonyms EIF4EL1, EIF4F
Function Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap.
Cellular Location Cytoplasm, P-body. Cytoplasm
Citations ( 0 )

Background

EIF4E recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures.

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$ 295.00
Cat# AM1852a
Size:
Quantity:
(40 western blots)
Availability: In Stock
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