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Nephrin Antibody (Ascites)

Mouse Monoclonal Antibody (Mab)

  • WB - Nephrin Antibody (Ascites) AM1865a
    Nephrin Antibody (C-term) (Cat. #AM1865a) western blot analysis in SK-BR-3 cell line lysates (15μg/lane).This demonstrates the Nephrin antibody detected the Nephrin protein (arrow).(1:100)
  • IF - Nephrin Antibody (Ascites) AM1865a
    Confocal immunofluorescent analysis of Nephrin Antibody (C-term) (Cat#AM1865a) with Hela cell followed by Alexa Fluor® 488-conjugated goat anti-mouse lgG (green). DAPI was used to stain the cell nuclear (blue).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O60500
Other Accession NP_004637.1
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgM,K
Clone Names 174CT2.1.1
Calculated MW 134742 Da
Antigen Region 1088-1117 aa
Additional Information
Gene ID 4868
Other Names Nephrin, Renal glomerulus-specific cell adhesion receptor, NPHS1, NPHN
Target/Specificity This Nephrin antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 1088-1117 amino acids from human Nephrin.
Dilution WB~~1:100~200
Format Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsNephrin Antibody (Ascites) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NPHS1
Synonyms NPHN
Function Seems to play a role in the development or function of the kidney glomerular filtration barrier. Regulates glomerular vascular permeability. May anchor the podocyte slit diaphragm to the actin cytoskeleton. Plays a role in skeletal muscle formation through regulation of myoblast fusion (By similarity).
Cellular Location Cell membrane; Single-pass type I membrane protein. Note=Predominantly located at podocyte slit diaphragm between podocyte foot processes. Also associated with podocyte apical plasma membrane
Tissue Location Specifically expressed in podocytes of kidney glomeruli
Research Areas
Citations (0)

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This gene encodes a member of the immunoglobulin family of cell adhesion molecules that functions in the glomerular filtration barrier in the kidney. The gene is primarily expressed in renal tissues, and the protein is a type-1 transmembrane protein found at the slit diaphragm of glomerular podocytes. The slit diaphragm is thought to function as an ultrafilter to exclude albumin and other plasma macromolecules in the formation of urine. Mutations in this gene result in Finnish-type congenital nephrosis 1, characterized by severe proteinuria and loss of the slit diaphragm and foot processes.


Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010) Wu, F., et al. J. Am. Soc. Nephrol. 21(9):1456-1467(2010) Tossidou, I., et al. J. Biol. Chem. 285(33):25285-25295(2010) Machuca, E., et al. J. Am. Soc. Nephrol. 21(7):1209-1217(2010) Aya, K., et al. Kidney Int. 57(2):401-404(2000)

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Cat# AM1865a
(40 western blots)
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