|Application ||WB, FC, E|
|Calculated MW||124074 Da|
|Antigen Region||69-97 aa|
|Other Names||Zinc finger E-box-binding homeobox 1, NIL-2-A zinc finger protein, Negative regulator of IL2, Transcription factor 8, TCF-8, ZEB1, AREB6, TCF8|
|Target/Specificity||This ZEB1 antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 69-97 amino acids from human ZEB1.|
|Format||Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||ZEB1 antibody ( Ascites) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Acts as a transcriptional repressor. Inhibits interleukin-2 (IL-2) gene expression. Enhances or represses the promoter activity of the ATP1A1 gene depending on the quantity of cDNA and on the cell type. Represses E-cadherin promoter and induces an epithelial-mesenchymal transition (EMT) by recruiting SMARCA4/BRG1. Represses BCL6 transcription in the presence of the corepressor CTBP1. Positively regulates neuronal differentiation. Represses RCOR1 transcription activation during neurogenesis. Represses transcription by binding to the E box (5'-CANNTG-3'). Promotes tumorigenicity by repressing stemness-inhibiting microRNAs.|
|Tissue Location||Colocalizes with SMARCA4/BRG1 in E-cadherin- negative cells from established lines, and stroma of normal colon as well as in de-differentiated epithelial cells at the invasion front of colorectal carcinomas (at protein level). Expressed in heart and skeletal muscle, but not in liver, spleen, or pancreas|
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This gene encodes a zinc finger transcription factor. The encoded protein likely plays a role in transcriptional repression of interleukin 2. Mutations in this gene have been associated with posterior polymorphous corneal dystrophy-3 and late-onset Fuchs endothelial corneal dystrophy. Alternatively spliced transcript variants encoding different isoforms have been described.
Drake, J.M., et al. J. Biol. Chem. 285(44):33940-33948(2010) Takeyama, Y., et al. Cancer Lett. 296(2):216-224(2010) Nakahata, S., et al. Oncogene 29(29):4157-4169(2010) Mehta, J.S., et al. Invest. Ophthalmol. Vis. Sci. 49(1):184-188(2008) Manavella, P.A., et al. Biochem. Biophys. Res. Commun. 360(3):621-626(2007)
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