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ATG4D AntibodyMouse Monoclonal Antibody (Mab)
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AM1896b | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
| AM1896b-ev20 | Ev20 size 100 ul | In Stock | $ 95.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
- Reviews
- Protocols
- Backgrounds
ATG4D Antibody - Product info | |
| Application | WB, IF
|
| Primary Accession | Q86TL0 |
| Other Accession | NP_116274.3 |
| Reactivity | Human |
| Concentration | 0.25 mg/ml |
| Isotype | IgG1,K |
| Clone Names | 222CT15.4.1 |
| Calculated MW | 52922 Da |
ATG4D Antibody - Additional info | |
| Gene ID 84971 | |
| Other Names ATG4D; APG4D; AUTL4; Cysteine protease ATG4D; AUT-like 4 cysteine endopeptidase; Autophagin-4; Autophagy-related cysteine endopeptidase 4; Autophagy-related protein 4 homolog D | |
| Target/Specificity This ATG4D monoclonal antibody is generated from mouse immunized with ATG4D recombinant protein. | |
| Dilution WB~~1:100~1000 IF~~1:10~50 | |
| Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions ATG4D Antibody is for research use only and not for use in diagnostic or therapeutic procedures. | |
ATG4D Antibody - Protein Information | |
| Name ATG4D | |
| Synonyms APG4D, AUTL4 | |
| Function Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes | |
| Cellular Location Cytoplasm (Probable). | |
| Tissue Location Mainly expressed in skeletal muscle and, to a lower extent, in testis | |
ATG4D Antibody - Related products
AM1896a: ATG4D antibody (Ascites)
AP11448a: ATG4D Antibody (N-term)
AP17051c: APG4D Antibody (Center S341)
AP1811a: ATG4D Antibody (N-term)
AP1811b: ATG4D Antibody (Center)
AP1811c: ATG4D Antibody (C-term)
AP1811e: ATG4D Antibody (C-term)
AP1811f: ATG4D Antibody (center)
AP3394a: Phospho-ATG4D-S15 Antibody
AP3395a: Phospho-ATG4D-S341 Antibody
AP3395b: Phospho-ATG4D-S467 Antibody
RI10457: ATG4D predesign siRNA
DC08667: Human ATG4D cDNA Clone
BP1811a: APG4D Antibody (N-term) Blocking Peptide
BP1811b: APG4D Antibody (Center) Blocking Peptide
BP1811c: APG4D Antibody (C-term) Blocking Peptide
BP1811e: APG4D Antibody (C-term) Blocking Peptide
BP1811f: ATG4D Antibody (center) Blocking Peptide
BP3394a: Phospho-APG4D-S15 Antibody Blocking Peptide
ATG4D Antibody - Application data
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ATG4D/MB10250 antibody (Cat. #AM1896b) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the ATG4D/MB10250 antibody detected the ATG4D/MB10250 protein (arrow).
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Confocal immunofluorescent analysis of ATG4D Antibody (Cat#AM1896b) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green). DAPI was used to stain the cell nuclear (blue).
ATG4D Antibody - Research Areas
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BACKGROUND
Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases.
REFERENCES
Betin, V.M., et al. J. Cell. Sci. 122 (PT 14), 2554-2566 (2009) : Lamesch, P., et al. Genomics 89(3):307-315(2007) Marino, G., et al. J. Biol. Chem. 278(6):3671-3678(2003)
