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TSC2 Antibody

Mouse Monoclonal Antibody (Mab)

  • IF - TSC2 Antibody AM1919B
    Fluorescent image of Hela cell stained with TSC2 Antibody(Cat#AM1919b/SG110509AA).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TSC2 primary antibody (1:25, 1 h at 37℃. For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-mouse antibody (green) was used (1:400, 50 min at 37℃.Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃. Nuclei were counterstained with DAPI (blue) (10 μg/ml, 10 min).TSC2 immunoreactivity is localized to Microtubules significantly.
  • WB - TSC2 Antibody AM1919B
    TSC2 Antibody (Cat. #AM1919b) western blot analysis in mouse NIH-3T3 cell line lysates (35μg/lane).This demonstrates the TSC2 antibody detected the TSC2 protein (arrow).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P49815
Other Accession NP_000539.2, NP_001070651.1
Reactivity Mouse
Host Mouse
Clonality Monoclonal
Isotype IgG1,k
Clone Names 249CT19.1.3
Calculated MW 200608 Da
Additional Information
Gene ID 7249
Other Names Tuberin, Tuberous sclerosis 2 protein, TSC2, TSC4
Target/Specificity This TSC2 monoclonal antibody is generated from mouse immunized with TSC2 recombinant protein.
Dilution IF~~1:10~50
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsTSC2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TSC2
Synonyms TSC4
Function In complex with TSC1, inhibits the nutrient-mediated or growth factor-stimulated phosphorylation of S6K1 and EIF4EBP1 by negatively regulating mTORC1 signaling. Acts as a GTPase- activating protein (GAP) for the small GTPase RHEB, a direct activator of the protein kinase activity of mTORC1. Implicated as a tumor suppressor. Involved in microtubule-mediated protein transport, but this seems to be due to unregulated mTOR signaling. Stimulates weakly the intrinsic GTPase activity of the Ras-related proteins RAP1A and RAB5 in vitro. Mutations in TSC2 lead to constitutive activation of RAP1A in tumors.
Cellular Location Cytoplasm. Membrane; Peripheral membrane protein. Note=At steady state found in association with membranes
Tissue Location Liver, brain, heart, lymphocytes, fibroblasts, biliary epithelium, pancreas, skeletal muscle, kidney, lung and placenta
Research Areas
Citations (0)

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Mutations in this gene lead to tuberous sclerosis complex. Its gene product is believed to be a tumor suppressor and is able to stimulate specific GTPases. The protein associates with hamartin in a cytosolic complex, possibly acting as a chaperone for hamartin. Alternative splicing results in multiple transcript variants encoding different isoforms.


References for protein:
1.Slattery, M.L., et al. Carcinogenesis 31(9):1604-1611(2010)
2.Larson, Y., et al. J. Biol. Chem. 285(32):24987-24998(2010)
3.Mehta, M.S., et al. Breast Cancer Res. Treat. (2010) In press :
4.Mieulet, V., et al. Trends Mol Med 16(7):329-335(2010)
5.Liu, C.Y., et al. Carcinogenesis 31(7):1259-1263(2010)

References for HeLa cell line:
1. Scherer WF, Syverton JT, Gey GO (May 1953). "Studies on the propagation in vitro of poliomyelitis viruses. IV. Viral multiplication in a stable strain of human malignant epithelial cells (strain HeLa) derived from an epidermoid carcinoma of the cervix". J. Exp. Med. 97 (5): 695–710. [PubMed:13052828].

2. Macville M, Schröck E, Padilla-Nash H, Keck C, Ghadimi BM, Zimonjic D, Popescu N, Ried T (January 1999). "Comprehensive and definitive molecular cytogenetic characterization of HeLa cells by spectral karyotyping". Cancer Res. 59 (1): 141–50. [PubMed: 9892199].
3. Rahbari R, Sheahan T, Modes V, Collier P, Macfarlane C, Badge RM (April 2009). "A novel L1 retrotransposon marker for HeLa cell line identification". BioTechniques 46 (4): 277–84. [PubMed: 19450234].

4. Capes-Davis A, Theodosopoulos G, Atkin I, Drexler HG, Kohara A, MacLeod RA, Masters JR, Nakamura Y, Reid YA, Reddel RR, Freshney RI (July 2010). "Check your cultures! A list of cross-contaminated or misidentified cell lines". Int. J. Cancer 127 (1): 1–8. [PubMed:20143388].

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Cat# AM1919B
(40 western blots)
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