|Application ||WB, E|
|Other Accession||Q684M4, NP_036421.2, NP_987096.1|
|Calculated MW||69666 Da|
|Antigen Region||422-449 aa|
|Other Names||Kelch-like ECH-associated protein 1, Cytosolic inhibitor of Nrf2, INrf2, Kelch-like protein 19, KEAP1, INRF2, KIAA0132, KLHL19|
|Target/Specificity||This KEAP1 antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 422-449 amino acids from human KEAP1.|
|Format||Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Euglobin precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||KEAP1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||INRF2, KIAA0132, KLHL19|
|Function||Acts as a substrate adapter protein for the E3 ubiquitin ligase complex formed by CUL3 and RBX1 and targets NFE2L2/NRF2 for ubiquitination and degradation by the proteasome, thus resulting in the suppression of its transcriptional activity and the repression of antioxidant response element-mediated detoxifying enzyme gene expression. Retains NFE2L2/NRF2 and may also retain BPTF in the cytosol. Targets PGAM5 for ubiquitination and degradation by the proteasome.|
|Cellular Location||Cytoplasm. Nucleus. Note=Shuttles between cytoplasm and nucleus|
|Tissue Location||Broadly expressed, with highest levels in skeletal muscle.|
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Provided below are standard protocols that you may find useful for product applications.
This gene encodes a protein containing KELCH-1 like domains, as well as a BTB/POZ domain. Kelch-like ECH-associated protein 1 interacts with NF-E2-related factor 2 in a redox-sensitive manner and the dissociation of the proteins in the cytoplasm is followed by transportation of NF-E2-related factor 2 to the nucleus. This interaction results in the expression of the catalytic subunit of gamma-glutamylcysteine synthetase. Two alternatively spliced transcript variants encoding the same isoform have been found for this gene.
Dinkova-Kostova, A.T., et al. J. Biol. Chem. 285(44):33747-33755(2010)
Kang, H.J., et al. J. Biol. Chem. 285(28):21258-21268(2010)
Lau, A., et al. Mol. Cell. Biol. 30(13):3275-3285(2010)
Copple, I.M., et al. J. Biol. Chem. 285(22):16782-16788(2010)
Takahashi, T., et al. J Surg Oncol 101(6):500-506(2010)
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