|Application ||WB, E|
|Other Accession||Q684M4, NP_036421.2, NP_987096.1|
|Calculated MW||69666 Da|
|Antigen Region||422-449 aa|
|Other Names||Kelch-like ECH-associated protein 1, Cytosolic inhibitor of Nrf2, INrf2, Kelch-like protein 19, KEAP1, INRF2, KIAA0132, KLHL19|
|Target/Specificity||This KEAP1 antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 422-449 amino acids from human KEAP1.|
|Format||Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Euglobin precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||KEAP1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||INRF2, KIAA0132, KLHL19|
|Function||Acts as a substrate adapter protein for the E3 ubiquitin ligase complex formed by CUL3 and RBX1 and targets NFE2L2/NRF2 for ubiquitination and degradation by the proteasome, thus resulting in the suppression of its transcriptional activity and the repression of antioxidant response element-mediated detoxifying enzyme gene expression. Retains NFE2L2/NRF2 and may also retain BPTF in the cytosol. Targets PGAM5 for ubiquitination and degradation by the proteasome.|
|Cellular Location||Cytoplasm. Nucleus. Note=Shuttles between cytoplasm and nucleus|
|Tissue Location||Broadly expressed, with highest levels in skeletal muscle.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
email@example.com, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
This gene encodes a protein containing KELCH-1 like domains, as well as a BTB/POZ domain. Kelch-like ECH-associated protein 1 interacts with NF-E2-related factor 2 in a redox-sensitive manner and the dissociation of the proteins in the cytoplasm is followed by transportation of NF-E2-related factor 2 to the nucleus. This interaction results in the expression of the catalytic subunit of gamma-glutamylcysteine synthetase. Two alternatively spliced transcript variants encoding the same isoform have been found for this gene.
Dinkova-Kostova, A.T., et al. J. Biol. Chem. 285(44):33747-33755(2010)
Kang, H.J., et al. J. Biol. Chem. 285(28):21258-21268(2010)
Lau, A., et al. Mol. Cell. Biol. 30(13):3275-3285(2010)
Copple, I.M., et al. J. Biol. Chem. 285(22):16782-16788(2010)
Takahashi, T., et al. J Surg Oncol 101(6):500-506(2010)
If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at firstname.lastname@example.org.