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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   AKT1S1 Antibody   

AKT1S1 Antibody

Mouse Monoclonal Antibody (Mab)

  • WB - AKT1S1 Antibody AM2018b
    AKT1S1 Antibody (Cat. #AM2018b) western blot analysis in A375 cell line lysates (35μg/lane).This demonstrates the AKT1S1 antibody detected the AKT1S1 protein (arrow).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q96B36
Other Accession NP_001092102.1
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names 449CT13.4.2
Calculated MW 27383 Da
Additional Information
Gene ID 84335
Other Names Proline-rich AKT1 substrate 1, 40 kDa proline-rich AKT substrate, AKT1S1 {ECO:0000312|EMBL:AAH160431}
Target/Specificity Purified His-tagged AKT1S1 protein(Fragment) was used to produced this monoclonal antibody.
Dilution WB~~1:100
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsAKT1S1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name AKT1S1 {ECO:0000312|EMBL:AAH16043.1}
Function Subunit of mTORC1, which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino acids. Growth factor-stimulated mTORC1 activation involves a AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Within mTORC1, AKT1S1 negatively regulates mTOR activity in a manner that is dependent on its phosphorylation state and binding to 14-3-3 proteins. Inhibits RHEB-GTP-dependent mTORC1 activation. Substrate for AKT1 phosphorylation, but can also be activated by AKT1-independent mechanisms. May also play a role in nerve growth factor-mediated neuroprotection.
Cellular Location Cytoplasm, cytosol. Note=Found in the cytosolic fraction of the brain.
Tissue Location Widely expressed with highest levels of expression in liver and heart. Expressed at higher levels in cancer cell lines (e.g. A-549 and HeLa) than in normal cell lines (e.g. HEK293).
Research Areas
Citations (0)

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AKT1S1 is a proline-rich substrate of AKT (MIM 164730) that binds 14-3-3 protein (see YWHAH, MIM 113508) when phosphorylated (Kovacina et al., 2003 [PubMed 12524439]).[supplied by OMIM].


Wang, L., et al. J. Biol. Chem. 283(23):15619-15627(2008)
Fonseca, B.D., et al. Biochem. J. 411(1):141-149(2008)
Fonseca, B.D., et al. J. Biol. Chem. 282(34):24514-24524(2007)
Wang, L., et al. J. Biol. Chem. 282(27):20036-20044(2007)
Sancak, Y., et al. Mol. Cell 25(6):903-915(2007)

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$ 295.00
$ 99.00
Cat# AM2018b
(40 western blots)
Availability: In Stock
Bulk Size
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