|Application ||WB, E|
|Calculated MW||85659 Da|
|Antigen Region||136-164 aa|
|Other Names||Glucocorticoid receptor, GR, Nuclear receptor subfamily 3 group C member 1, NR3C1, GRL|
|Target/Specificity||This NR3C1 antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 136-164 amino acids from human NR3C1.|
|Format||Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Euglobin precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||NR3C1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Receptor for glucocorticoids (GC) (PubMed:27120390). Has a dual mode of action: as a transcription factor that binds to glucocorticoid response elements (GRE), both for nuclear and mitochondrial DNA, and as a modulator of other transcription factors. Affects inflammatory responses, cellular proliferation and differentiation in target tissues. Involved in chromatin remodeling (PubMed:9590696). Plays a role in rapid mRNA degradation by binding to the 5' UTR of target mRNAs and interacting with PNRC2 in a ligand-dependent manner which recruits the RNA helicase UPF1 and the mRNA-decapping enzyme DCP1A, leading to RNA decay (PubMed:25775514). Could act as a coactivator for STAT5-dependent transcription upon growth hormone (GH) stimulation and could reveal an essential role of hepatic GR in the control of body growth (By similarity).|
|Cellular Location||Isoform Alpha: Cytoplasm. Nucleus Mitochondrion. Cytoplasm, cytoskeleton, spindle. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Note=After ligand activation, translocates from the cytoplasm to the nucleus Isoform Alpha-B: Nucleus. Cytoplasm. Note=After ligand activation, translocates from the cytoplasm to the nucleus|
|Tissue Location||Widely expressed including bone, stomach, lung, liver, colon, breast, ovary, pancreas and kidney (PubMed:25847991). In the heart, detected in left and right atria, left and right ventricles, aorta, apex, intraventricular septum, and atrioventricular node as well as whole adult and fetal heart (PubMed:10902803). Isoform Beta: Widely expressed including brain, bone marrow, thymus, spleen, liver, kidney, pancreas, lung, fat, skeletal muscle, heart, placenta and blood leukocytes (PubMed:7769088, PubMed:8621628). Isoform Alpha-2: Expressed at low level.|
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Provided below are standard protocols that you may find useful for product applications.
Receptor for glucocorticoids (GC). Has a dual mode of action: as a transcription factor that binds to glucocorticoid response elements (GRE) and as a modulator of other transcription factors. Affects inflammatory responses, cellular proliferation and differentiation in target tissues. Could act as a coactivator for STAT5-dependent transcription upon growth hormone (GH) stimulation and could reveal an essential role of hepatic GR in the control of body growth. Involved in chromatin remodeling. Plays a significant role in transactivation. Involved in nuclear translocation (By similarity).
Hollenberg S.M., et al. Nature 318:635-641(1985).
Encio I.J., et al. J. Biol. Chem. 266:7182-7188(1991).
Wang W., et al. Nucleic Acids Res. 39:44-58(2011).
Turner J.D., et al. Ann. N. Y. Acad. Sci. 1095:334-341(2007).
Munroe D.G., et al. Submitted (SEP-1993) to the EMBL/GenBank/DDBJ databases.
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