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SUMO2 Antibody (C-term)
Mouse Monoclonal Antibody (Mab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| IF, WB, E |
|---|---|
| Primary Accession | P61956 |
| Other Accession | P61959, P61958, P61957, Q2PFW2, Q6LDZ8, Q5ZJM9, P61955 |
| Reactivity | Human, Rat |
| Predicted | Bovine, Chicken, Hamster, Monkey, Mouse, Pig |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2b |
| Clone/Animal Names | 973CT8.1.1 |
| Calculated MW | 10871 Da |
| Gene ID | 6613 |
|---|---|
| Other Names | Small ubiquitin-related modifier 2, SUMO-2, HSMT3, SMT3 homolog 2 {ECO:0000312|HGNC:HGNC:11125}, SUMO-3, Sentrin-2, Ubiquitin-like protein SMT3B, Smt3B, SUMO2 (HGNC:11125) |
| Target/Specificity | Purified His-tagged SUMO2 protein was used to produced this monoclonal antibody. |
| Dilution | IF~~1:25 WB~~1:1000 |
| Format | Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | SUMO2 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | SUMO2 (HGNC:11125) |
|---|---|
| Function | Ubiquitin-like protein that can be covalently attached to proteins as a monomer or as a lysine-linked polymer. Covalent attachment via an isopeptide bond to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by an E3 ligase such as PIAS1-4, RANBP2, CBX4 or ZNF451 (PubMed:26524494). This post-translational modification on lysine residues of proteins plays a crucial role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Polymeric SUMO2 chains are also susceptible to polyubiquitination which functions as a signal for proteasomal degradation of modified proteins (PubMed:18408734, PubMed:18538659, PubMed:21965678, PubMed:9556629). Plays a role in the regulation of sumoylation status of SETX (PubMed:24105744). |
| Cellular Location | Nucleus. Nucleus, PML body. |
| Tissue Location | Broadly expressed.. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Ubiquitin-like protein that can be covalently attached to proteins as a monomer or as a lysine-linked polymer. Covalent attachment via an isopeptide bond to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by an E3 ligase such as PIAS1-4, RANBP2 or CBX4. This post-translational modification on lysine residues of proteins plays a crucial role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Polymeric SUMO2 chains are also susceptible to polyubiquitination which functions as a signal for proteasomal degradation of modified proteins.
References
Reverter D., et al. Structure 12:1519-1531(2004).
Xu Z., et al. Biochem. J. 386:325-330(2005).
Mannen H., et al. Biochem. Biophys. Res. Commun. 222:178-180(1996).
Lapenta V., et al. Genomics 40:362-367(1997).
Ota T., et al. Nat. Genet. 36:40-45(2004).
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