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CASP3 Antibody

Mouse Monoclonal Antibody (Mab)

     
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  • WB - CASP3 Antibody AM2239b
    CASP3 Antibody (Cat. #AM2239b) western blot analysis in Jurkat,Ramos cell line lysates (35μg/lane).This demonstrates the CASP3 antibody detected the CASP3 protein (arrow).
  • SPECIFICATION
  • CITATIONS: 1
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession P42574
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names 1262CT521.280.101
Calculated MW 31608 Da
Additional Information
Gene ID 836
Other Names Caspase-3, CASP-3, Apopain, Cysteine protease CPP32, CPP-32, Protein Yama, SREBP cleavage activity 1, SCA-1, Caspase-3 subunit p17, Caspase-3 subunit p12, CASP3, CPP32
Target/Specificity Purified His-tagged CASP3 protein was used to produced this monoclonal antibody.
Dilution WB~~1:2000
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCASP3 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CASP3
Synonyms CPP32
Function Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop- helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage.
Cellular Location Cytoplasm.
Tissue Location Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system
Research Areas
Citations ( 0 )

Background

Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage.

References

Choudhary C., et al. Science 325:834-840(2009).
Fernandes-Alnemri T., et al. J. Biol. Chem. 269:30761-30764(1994).
Tewari M., et al. Cell 81:801-809(1995).
Pelletier M., et al. Biochem. Biophys. Res. Commun. 316:93-99(2004).
Ota T., et al. Nat. Genet. 36:40-45(2004).

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$ 295.00
$ 99.00
Cat# AM2239b
Size:
Quantity:
(40 western blots)
Availability: In Stock
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