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>   home   >   Products   >   Primary Antibodies   >   Cell Biology   >   USP11 Antibody (C-term R565)   

USP11 Antibody (C-term R565)

Purified Mouse Monoclonal Antibody (Mab)

     
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  • FC - USP11 Antibody (C-term R565) AM2260b
    Flow cytometric analysis of Hela cells using USP11 Antibody (C-term R565)(green, Cat#AM2260b) compared to an isotype control of mouse IgG1(blue). AM2260b was diluted at 1:25 dilution. An Alexa Fluor® 488 goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody.
  • WB - USP11 Antibody (C-term R565) AM2260b
    Western blot analysis of lysates from Jurkat, Hela, LNCaP cell line (from left to right) using USP11 Antibody (N-term) (Cat. # AM2260b). AM2260b was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysates at 35μg per lane.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, FC, E
Primary Accession P51784
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG1,κ
Clone Names 1220CT620.193.189
Calculated MW 109817 Da
Additional Information
Gene ID 8237
Other Names Ubiquitin carboxyl-terminal hydrolase 11, Deubiquitinating enzyme 11, Ubiquitin thioesterase 11, Ubiquitin-specific-processing protease 11, USP11, UHX1
Target/Specificity This USP11 antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between 32-300 amino acids from the N-terminal region of human USP11.
Dilution FC~~1:25
WB~~1:1000
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsUSP11 Antibody (C-term R565) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name USP11
Synonyms UHX1
Function Protease that can remove conjugated ubiquitin from target proteins and polyubiquitin chains. Inhibits the degradation of target proteins by the proteasome. Plays a role in the regulation of pathways leading to NF-kappa-B activation. Plays a role in the regulation of DNA repair after double-stranded DNA breaks.
Cellular Location Nucleus. Cytoplasm. Note=Predominantly nuclear. Associates with chromatin
Research Areas
Citations (0)

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Background

Protease that can remove conjugated ubiquitin from target proteins and polyubiquitin chains. Inhibits the degradation of target proteins by the proteasome. Plays a role in the regulation of pathways leading to NF-kappa-B activation. Plays a role in the regulation of DNA repair after double-stranded DNA breaks.

References

Ross M.T.,et al.Nature 434:325-337(2005).
Ideguchi H.,et al.Biochem. J. 367:87-95(2002).
Swanson D.A.,et al.Hum. Mol. Genet. 5:533-538(1996).
Schoenfeld A.R.,et al.Mol. Cell. Biol. 24:7444-7455(2004).
Yamaguchi T.,et al.J. Biol. Chem. 282:33943-33948(2007).

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$ 295.00
$ 99.00
Cat# AM2260b
Size:
Quantity:
(40 western blots)
Availability: In Stock
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