|Application ||WB, FC, E|
|Calculated MW||109817 Da|
|Other Names||Ubiquitin carboxyl-terminal hydrolase 11, Deubiquitinating enzyme 11, Ubiquitin thioesterase 11, Ubiquitin-specific-processing protease 11, USP11, UHX1|
|Target/Specificity||This USP11 antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between 32-300 amino acids from the N-terminal region of human USP11.|
|Format||Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||USP11 Antibody (C-term R565) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Protease that can remove conjugated ubiquitin from target proteins and polyubiquitin chains (PubMed:12084015, PubMed:15314155, PubMed:17897950, PubMed:19874889, PubMed:20233726, PubMed:24724799). Inhibits the degradation of target proteins by the proteasome (PubMed:12084015). Cleaves preferentially 'Lys-6' and 'Lys-63'-linked ubiquitin chains. Has lower activity with 'Lys-11' and 'Lys-33'-linked ubiquitin chains, and extremely low activity with 'Lys-27', 'Lys-29' and 'Lys-48'- linked ubiquitin chains (in vitro) (PubMed:24724799). Plays a role in the regulation of pathways leading to NF-kappa-B activation (PubMed:17897950, PubMed:19874889). Plays a role in the regulation of DNA repair after double-stranded DNA breaks (PubMed:15314155, PubMed:20233726). Acts as a chromatin regulator via its association with the Polycomb group (PcG) multiprotein PRC1-like complex; may act by deubiquitinating components of the PRC1-like complex (PubMed:20601937).|
|Cellular Location||Nucleus Cytoplasm. Chromosome Note=Predominantly nuclear (PubMed:12084015, PubMed:15314155) Associates with chromatin (PubMed:20601937, PubMed:20233726)|
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Provided below are standard protocols that you may find useful for product applications.
Protease that can remove conjugated ubiquitin from target proteins and polyubiquitin chains. Inhibits the degradation of target proteins by the proteasome. Plays a role in the regulation of pathways leading to NF-kappa-B activation. Plays a role in the regulation of DNA repair after double-stranded DNA breaks.
Ross M.T.,et al.Nature 434:325-337(2005).
Ideguchi H.,et al.Biochem. J. 367:87-95(2002).
Swanson D.A.,et al.Hum. Mol. Genet. 5:533-538(1996).
Schoenfeld A.R.,et al.Mol. Cell. Biol. 24:7444-7455(2004).
Yamaguchi T.,et al.J. Biol. Chem. 282:33943-33948(2007).
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