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PAH Antibody (Center)

Purified Mouse Monoclonal Antibody (Mab)

     
  • IHC-P - PAH Antibody (Center) AM8419b
    AM8419b staining PAH in human kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
  • IHC-P - PAH Antibody (Center) AM8419b
    Immunohistochemical analysis of paraffin-embedded H. liver section using PAH Antibody (Center)(Cat#AM8419b). AM8419b was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
  • IHC-P - PAH Antibody (Center) AM8419b
    Immunohistochemical analysis of paraffin-embedded M. kidney section using PAH Antibody (Center)(Cat#AM8419b). AM8419b was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
  • WB - PAH Antibody (Center) AM8419b
    Western blot analysis of lysates from HepG2 cell line and mouse liver rat liver tissue lysates (from left to right) using PAH Antibody (Center)(Cat. #AM8419b). AM8419b was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysates at 35μg per lane.
  • SPECIFICATION
  • CITATIONS
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IHC-P, WB, E
Primary Accession P00439
Other Accession P04176, P16331, Q2KIH7
Reactivity Human, Mouse, Rat
Predicted Bovine
Host Mouse
Clonality Monoclonal
Isotype IgG1,κ
Clone Names 717CT21.1.1
Calculated MW 51862 Da
Additional Information
Gene ID 5053
Other Names Phenylalanine-4-hydroxylase, PAH, Phe-4-monooxygenase, PAH
Target/Specificity This PAH antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between 127-161 amino acids from the Central region of human PAH.
Dilution IHC-P~~1:25
WB~~1:1000
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPAH Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PAH
Research Areas
Citations (0)

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References

Kwok S.C.M.,et al.Biochemistry 24:556-561(1985).
Scriver C.R.,et al.Submitted (SEP-1997) to the EMBL/GenBank/DDBJ databases.
Cotton R.G.,et al.Biochem. J. 255:193-196(1988).
Miranda F.F.,et al.J. Biol. Chem. 277:40937-40943(2002).
Siltberg-Liberles J.,et al.Gene 427:86-92(2008).

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$ 295.00
$ 99.00
Cat# AM8419b
Size:
Quantity:
(40 western blots)
Availability: In Stock
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