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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   RAB3B Antibody   

RAB3B Antibody

Purified Mouse Monoclonal Antibody (Mab)

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  • FC - RAB3B Antibody AM8487b
    Overlay histogram showing HepG2 cells stained with AM8487b (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AM8487b, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(NA168821) at 1/400 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was mouse IgG (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • IHC-P - RAB3B Antibody AM8487b
    AM8487b staining RAB3B in human pancreas sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
  • WB - RAB3B Antibody AM8487b
    All lanes : Anti-RAB3B Antibody at 1:2000 dilution Lane 1: mouse brain lysate Lane 2: rat brain lysate Lane 3: SW480 whole cell lysate Lane 4: human brain lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 25 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P20337
Reactivity Human, Mouse, Rat
Host Mouse
Clonality monoclonal
Isotype IgG1,k
Clone Names 1543CT354.60.92
Calculated MW 24758 Da
Additional Information
Gene ID 5865
Other Names Ras-related protein Rab-3B, RAB3B
Target/Specificity This RAB3B antibody is generated from a mouse immunized with a recombinant protein of human RAB3B.
Dilution FC~~1:25
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRAB3B Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RAB3B
Function Protein transport. Probably involved in vesicular traffic (By similarity).
Cellular Location Cell membrane; Lipid-anchor; Cytoplasmic side
Research Areas
Citations (0)

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Protein transport. Probably involved in vesicular traffic (By similarity).


Zahraoui A.,et al.J. Biol. Chem. 264:12394-12401(1989).
Puhl H.L. III,et al.Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases.
Gregory S.G.,et al.Nature 441:315-321(2006).
Rigbolt K.T.,et al.Sci. Signal. 4:RS3-RS3(2011).

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$ 295.00
$ 99.00
Cat# AM8487b
(40 western blots)
Availability: In Stock
Bulk Size
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