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>   home   >   Products   >   Primary Antibodies   >   Cell Biology   >   MB21D1 Antibody   

MB21D1 Antibody

Purified Mouse Monoclonal Antibody (Mab)

     
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  • WB - MB21D1 Antibody AM8562b
    All lanes : Anti-MB21D1 Antibody at 1:4000 dilution Lane 1: THP-1 whole cell lysate Lane 2: MDA-MB-231 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 59kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q8N884
Reactivity Human
Host Mouse
Clonality monoclonal
Isotype IgG1,k
Clone Names 1697CT136.65.30
Calculated MW 58814 Da
Additional Information
Gene ID 115004
Other Names Cyclic GMP-AMP synthase, cGAMP synthase, cGAS, h-cGAS, 2.7.7.86, Mab-21 domain-containing protein 1, MB21D1, C6orf150
Target/Specificity This MB21D1 antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between 1-185 amino acids from human MB21D1.
Dilution WB~~1:4000
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMB21D1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MB21D1
Synonyms C6orf150
Function Nucleotidyltransferase that catalyzes the formation of cyclic GMP-AMP (cGAMP) from ATP and GTP. Catalysis involves both the formation of a 2',5' phosphodiester linkage at the GpA step and the formation of a 3',5' phosphodiester linkage at the ApG step, producing c[G(2',5')pA(3',5')p]. Has antiviral activity by acting as a key cytosolic DNA sensor, the presence of double- stranded DNA (dsDNA) in the cytoplasm being a danger signal that triggers the immune responses. Binds cytosolic DNA directly, leading to activation and synthesis of cGAMP, a second messenger that binds to and activates TMEM173/STING, thereby triggering type-I interferon production.
Cellular Location Cytoplasm, cytosol
Tissue Location Expressed in the monocytic cell line THP1.
Research Areas
Citations (0)

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Background

Nucleotidyltransferase that catalyzes the formation of cyclic GMP-AMP (cGAMP) from ATP and GTP. Catalysis involves both the formation of a 2',5' phosphodiester linkage at the GpA step and the formation of a 3',5' phosphodiester linkage at the ApG step, producing c[G(2',5')pA(3',5')p]. Has antiviral activity by acting as a key cytosolic DNA sensor, the presence of double- stranded DNA (dsDNA) in the cytoplasm being a danger signal that triggers the immune responses. Binds cytosolic DNA directly, leading to activation and synthesis of cGAMP, a second messenger that binds to and activates TMEM173/STING, thereby triggering type-I interferon production.

References

Sun L.,et al.Science 339:786-791(2013).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Mungall A.J.,et al.Nature 425:805-811(2003).
Choudhary C.,et al.Science 325:834-840(2009).
Olsen J.V.,et al.Sci. Signal. 3:RA3-RA3(2010).

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$ 295.00
$ 99.00
Cat# AM8562b
Size:
Quantity:
(40 western blots)
Availability: In Stock
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