|Application ||WB, E|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||125216 Da|
|Other Names||Eukaryotic translation initiation factor 2-alpha kinase 3, 220.127.116.11, PRKR-like endoplasmic reticulum kinase, Pancreatic eIF2-alpha kinase, HsPEK, EIF2AK3, PEK, PERK|
|Target/Specificity||This EIF2AK3 antibody is generated from a mouse immunized with a recombinant protein conjugated synthetic peptide between 530-850 amino acids from human EIF2AK3.|
|Format||Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||EIF2AK3 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Metabolic-stress sensing protein kinase that phosphorylates the alpha subunit of eukaryotic translation initiation factor 2 (eIF-2-alpha/EIF2S1) on 'Ser-52' during the unfolded protein response (UPR) and in response to low amino acid availability. Converts phosphorylated eIF-2-alpha/EIF2S1 either in a global protein synthesis inhibitor, leading to a reduced overall utilization of amino acids, or to a translation initiation activator of specific mRNAs, such as the transcriptional activator ATF4, and hence allowing ATF4-mediated reprogramming of amino acid biosynthetic gene expression to alleviate nutrient depletion. Serves as a critical effector of unfolded protein response (UPR)- induced G1 growth arrest due to the loss of cyclin-D1 (CCND1). Involved in control of mitochondrial morphology and function.|
|Cellular Location||Endoplasmic reticulum membrane; Single-pass type I membrane protein|
|Tissue Location||Ubiquitous. A high level expression is seen in secretory tissues|
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Provided below are standard protocols that you may find useful for product applications.
Phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2 (EIF2), leading to its inactivation and thus to a rapid reduction of translational initiation and repression of global protein synthesis. Serves as a critical effector of unfolded protein response (UPR)-induced G1 growth arrest due to the loss of cyclin-D1 (CCND1). Involved in control of mitochondrial morphology and function (By similarity).
Shi Y.,et al.J. Biol. Chem. 274:5723-5730(1999).
Sood R.,et al.Biochem. J. 346:281-293(2000).
Delepine M.,et al.Nat. Genet. 25:406-409(2000).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Hillier L.W.,et al.Nature 434:724-731(2005).
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