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>   home   >   Products   >   Primary Antibodies   >   Stem Cells   >   SMURF2 Antibody   

SMURF2 Antibody

Purified Mouse Monoclonal Antibody (Mab)

     
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  • WB - SMURF2 Antibody AM8633b
    All lanes : Anti-SMURF2 Antibody at 1:2000-1:4000 dilution Lane 1: MDA-MB-231 whole cell lysate Lane 2: U-2OS whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 86 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q9HAU4
Reactivity Human
Host Mouse
Clonality monoclonal
Isotype IgG1,k
Clone Names 1826CT865.51.28
Calculated MW 86196 Da
Additional Information
Gene ID 64750
Other Names E3 ubiquitin-protein ligase SMURF2, hSMURF2, 6.3.2.-, SMAD ubiquitination regulatory factor 2, SMAD-specific E3 ubiquitin-protein ligase 2, SMURF2
Target/Specificity This SMURF2 antibody is generated from a mouse immunized with a recombinant protein of human SMURF2.
Dilution WB~~1:2000-1:4000
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSMURF2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SMURF2
Function E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Interacts with SMAD1 and SMAD7 in order to trigger their ubiquitination and proteasome-dependent degradation. In addition, interaction with SMAD7 activates autocatalytic degradation, which is prevented by interaction with SCYE1. Forms a stable complex with the TGF-beta receptor-mediated phosphorylated SMAD2 and SMAD3. In this way, SMAD2 may recruit substrates, such as SNON, for ubiquitin-mediated degradation. Enhances the inhibitory activity of SMAD7 and reduces the transcriptional activity of SMAD2. Coexpression of SMURF2 with SMAD1 results in considerable decrease in steady-state level of SMAD1 protein and a smaller decrease of SMAD2 level.
Cellular Location Nucleus. Cytoplasm. Cell membrane. Membrane raft. Note=Cytoplasmic in the presence of SMAD7. Colocalizes with CAV1, SMAD7 and TGF-beta receptor in membrane rafts
Tissue Location Widely expressed.
Research Areas
Citations (0)

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Background

E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Interacts with SMAD1 and SMAD7 in order to trigger their ubiquitination and proteasome-dependent degradation. In addition, interaction with SMAD7 activates autocatalytic degradation, which is prevented by interaction with SCYE1. Forms a stable complex with the TGF-beta receptor-mediated phosphorylated SMAD2 and SMAD3. In this way, SMAD2 may recruit substrates, such as SNON, for ubiquitin-mediated degradation. Enhances the inhibitory activity of SMAD7 and reduces the transcriptional activity of SMAD2. Coexpression of SMURF2 with SMAD1 results in considerable decrease in steady-state level of SMAD1 protein and a smaller decrease of SMAD2 level.

References

Kavsak P.,et al.Mol. Cell 6:1365-1375(2000).
Lin X.,et al.J. Biol. Chem. 275:36818-36822(2000).
Zhang Y.,et al.Proc. Natl. Acad. Sci. U.S.A. 98:974-979(2001).
Bonni S.,et al.Nat. Cell Biol. 3:587-595(2001).
Di Guglielmo G.M.,et al.Nat. Cell Biol. 5:410-421(2003).

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$ 295.00
$ 99.00
Cat# AM8633b
Size:
Quantity:
(40 western blots)
Availability: In Stock
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