|Predicted||Bovine, Chicken, Human, Mouse, Xenopus, Zebrafish|
|Calculated MW||87 KDa|
|Other Names||Myristoylated alanine-rich C-kinase substrate, MARCKS, Protein kinase C substrate 80 kDa protein, Marcks, Macs|
|Target/Specificity||Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser152/156 conjugated to KLH.|
|Format||Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.|
|Antibody Specificity||Specific for the ~87k MARCKS protein phosphorylated at Ser152 and Ser156 in Western blots. Immunolabeling is blocked by the phosphopeptide used as the antigen but not by the corresponding dephosphopeptide. The immunolabeling is completely eliminated by λ-phosphatase|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Phospho-Ser152,156 MARCKS Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
Myristoylated Alanine-Rich C Kinase Substrate (MARCKS) is a major substrate for phosphorylation by protein kinase C (PKC) (Ouimet et al., 1990). The phosphorylation of Ser152/156 can be used as a measure of PKC activation although these sites are also phosphorylated by PRK1 (Palmer et al., 1996) MARCKS is a member of a family of calmodulin binding proteins and phosphorylation of Ser152/156 modulates the binding of MARCKS to calmodulin (Verghese et al., 1994).
Ouimet CC, Wang JKT, Walaas SI, Albert KA, Greengard P (1990) Localization of the MARCKS (87 kDa) protein, a major specific substrate for protein kinase C, in rat brain. J Neurosci 10:1683-1698.
Palmer RH, Schönwasser DC, Rahman D, Pappin DJC, Herget T, Parker PJ (1996) PRK1 phosphorylates MARCKS at the PKC sites: Serine 152, serine 156 and serine 163. FEBS Lett 378:281-285.
Verghese GM, Johnson JD, Vasulka C, Haupt DM, Stumpo DJ, Blackshear PJ (1994) Protein kinase C-mediated phosphorylation and calmodulin binding of recombinant myristoylated alanine-rich C kinase substrate (MARCKS) and MARCKS-related protein. J Biol Chem 269:9361-9367.
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