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>   home   >   Products   >   Primary Antibodies   >   Antibody Collections   >   Phospho Antibodies   >   Metabotropic Glutamate Receptor 2/3 Antibody   

Metabotropic Glutamate Receptor 2/3 Antibody

Affinity purified rabbit polyclonal antibody

     
  • IHC - Metabotropic Glutamate Receptor 2/3 Antibody AN1048
    Immunohistochemical analysis of paraffin-embedded R. brain section using Metabotropic Glutamate Receptor 2/3 Antibody (Cat#AN1048). AN1048 was diluted at 1:500 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
  • IHC - Metabotropic Glutamate Receptor 2/3 Antibody AN1048
    Immunohistochemical analysis of paraffin-embedded H. brain section using Metabotropic Glutamate Receptor 2/3 Antibody (Cat#AN1048). AN1048 was diluted at 1:500 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
  • IHC - Metabotropic Glutamate Receptor 2/3 Antibody AN1048
    Immunohistochemical analysis of paraffin-embedded M. brain section using Metabotropic Glutamate Receptor 2/3 Antibody (Cat#AN1048). AN1048 was diluted at 1:500 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
  • WB - Metabotropic Glutamate Receptor 2/3 Antibody AN1048
    Western blot of 10 ug of rat hippocampal lysate showing the specific immunolabeling of the ~110k monomer and the ~220k dimer of mGluR2 and mGluR3.
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC
Primary Accession P31421
Reactivity Rat
Predicted Bovine, Human, Mouse, Monkey, Zebrafish
Host Rabbit
Clonality polyclonal
Calculated MW 110/220 KDa
Additional Information
Gene ID 24415
Gene Name GRM2/3
Other Names Metabotropic glutamate receptor 2, mGluR2, Grm2, Gprc1b, Mglur2
Target/Specificity Synthetic peptide corresponding to amino acid residues from the C-terminal region conjugated to KLH.
Dilution IHC~~1:500
WB~~ 1:1000
Format Prepared from rabbit serum by affinity purification using a column to which the peptide immunogen was coupled.
Antibody Specificity Specific for the ~110k monomer and the ~220k dimer of mGluR2 and mGluR3. Immunolabeling is blocked by preadsorption of antibody with the peptide used as antigen to generate the antibody.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMetabotropic Glutamate Receptor 2/3 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
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Research Areas
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Background

The metabotropic glutamate receptors (mGluRs) are key receptors in the modulation of excitatory synaptic transmission in the central nervous system. They are implicated in many forms of neural plasticity as well as learning and memory and drug abuse (Bhattacharya et al., 2004; Francesconi et al., 2004; Wilson and Nicoll, 2001). Group I metabotropic glutamate receptors (consisting of mGluR1 and mGluR5) are G-protein-coupled neurotransmitter receptors that are localized in the perisynaptic region of the postsynaptic membrane. When activated, Group I mGluRs lead to stimulation of phospholipase and activation of Protein Kinase C. In contrast, activation of Group II metabotropic receptors (mGluR2 and mGluR3) leads to inhibition of adenylate cyclase. The mGluR2 subunit has been shown to be required for long-term potentiation at the mossy fiber input in the hippocampus (Yokoi et al., 1996).

References

Bhattacharya M, Babwah AV, Godin C, Anborgh PH, Dale LB, Poulter MO, Ferguson SSG (2004) Ral and phospholipase D2-dependent pathway for constitutive metabotropic glutamate receptor endocytosis. J Neurosci 24:8752-8761.
Francesconi W, Cammalleri M, Sanna PP (2004) The metabotropic glutamate receptor 5 is necessary for late-phase long-term potentiation in the hippocampal CA1 region. Brain Res 1022:12-18.
Wilson RI, Nicoll RA (2001) Endogenous cannabinoids mediate retrograde signalling at hippocampal synapses. Nature (London) 410:588-592.
Yokoi M, Kobayashi K, Manabe T, Takahashi T, Sakaguchi I, Katsuura G, Shigemoto R, Ohishi H, Nomura S, Nakamura K, Nakao K, Katsuki M, Nakanishi S (1996) Impairment of hippocampal mossy fiber LTD in mice lacking mGluR2. Science 273:645-647.

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$ 335.00
Cat# AN1048
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