|Reactivity||Bovine, Human, Pig, Rabbit|
|Calculated MW||55 KDa|
|Other Names||Aromatic-L-amino-acid decarboxylase, AADC, DOPA decarboxylase, DDC, DDC, AADC|
|Target/Specificity||Synthetic peptide corresponding to amino acid residues from the N-terminal region conjugated to KLH.|
|Format||Prepared from rabbit serum by affinity purification using an AminoLink® Plus column matrix to which purified, recombinant bovine DDC was coupled.|
|Antibody Specificity||Specific for the ~55k DDC protein.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||DOPA Decarboxylase Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
DOPA decarboxylase (aromatic L-amino acid decarboxylase, AADC; DDC) catalyzes the second reaction in the biosynthesis of catecholamines and serotonin (Waymire and Haycock, 2002; Berry et al., 1996; Haycock et al., 2003). It is also involved in the biosynthesis of trace amines. DDC antibodies can therefore be used as markers for dopaminergic, noradrenergic and serotonergic neurons in a variety of applications including depression, schizophrenia, Parkinson’s disease and drug abuse (Kish et al., 2001; Zhu et al., 2000; Zhu et al., 1999).
Berry MD, Juorio AV, Li XM, Boulton AA (1996) Aromatic L-amino acid decarboxylase: a neglected and misunderstood enzyme. Neurochem Res 21:1075-1087.
Haycock JW, Becker L, Ang L, Furukawa Y, Hornykiewicz O, Kish SJ (2003) Marked disparity between age-related changes in dopamine and other presynaptic dopaminergic markers in human striatum. J Neurochem 87:574-585.
Kish SJ, Kalasinsky KS, Derkach P, Schmunk GA, Guttman M, Ang L, Adams V, Furukawa Y, Haycock JW (2001) Striatal dopaminergic and serotonergic markers in human heroin users. Neuropsychopharmacology 24:561-567.
Waymire JC, Haycock JW (2002) Lack of regulation of aromatic L-amino acid decarboxylase in intact bovine chromaffin cells. J Neurochem 81:589-593.
Zhu MY, Klimek V, Haycock JW, Ordway GA (2000) Quantitation of tyrosine hydroxylase protein in the locus coeruleus from postmortem human brain. J Neurosci Meth 99:37-44.
Zhu MY, Klimek V, Dilley GE, Haycock JW, Stockmeier C, Overholser JC, Meltzer HY, Ordway GA (1999) Elevated levels of tyrosine hydroxylase in the locus coeruleus in major depression. Biol Psychiatry 46:1275-1286.
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