|Predicted||Bovine, Human, Mouse|
|Calculated MW||68/70 KDa|
|Other Names||Serine/threonine-protein kinase PAK 1, Alpha-PAK, Protein kinase MUK2, p21-activated kinase 1, PAK-1, p68-PAK, Pak1|
|Target/Specificity||Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr402 conjugated to KLH.|
|Format||Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.|
|Antibody Specificity||Specific for the ~68k to ~70k PAK protein phosphorylated at Thr402. Theimmunolabeling of PAK is completely eliminated by λ-phosphatase treatment.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Phospho-Thr402 PAK-1,2,3 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
In mammals, there are several identified isoforms of p21-activated protein kinases or PAKs: α-PAK (also known as PAK-1) and β-PAK (also known as PAK-3) are mostly brain-specific, while -PAK (also known as PAK-2) is expressed ubiquitously (Jakobi et al., 2003). Mutations of the gene coding for PAK-3 are associated with X-linked mental retardation and recent work indicates that PAK-3 is a key regulator of synapse formation and plasticity in the hippocampus (Boda et al., 2004). PAK-3 is thought to play a key role in regulation of cell shape and motility as well as cell death (Jakobi et al., 2003; Walter et al., 1998). Autophosphorylation of Thr402 in the protein has been found to be essential for activation of PAK (Jakobi et al., 2000).
Boda B, Alberi S, Nikonenko I, Node-Langlois R, Jourdain P, Moosmayer M, Parisi-Jourdain L, Muller D (2004) The
mental retardation protein PAK-3 contributes to synapse formation and plasticity in hippocampus. J Neurosci
Jakobi R, Huang Z, Walter BN, Tuazon PT, Traugh JA (2000) Substrates enhance autophosphorylation and
activation of p21-activated protein kinase gamma-PAK in the absence of activation loop phosphorylation. Eur J
Jakobi R, McCarthy CC, Koeppel MA, Stringer DK (2003) Caspase-activated PAK-2 is regulated by subcellular
targeting and proteasomal degradation. J Biol Chem 278:38675-38685.
Walter BN, Huang Z, Jakobi R, Tuazon PT, Alnemri ES, Litwack G, Traugh JA (1998) Cleavage and activation of
p21-activated protein kinase gamma-PAK by CPP32 (caspase 3). Effects of autophosphorylation on activity. J
Biol Chem 273:28733-28739.
Jia Nie, Chao Sun, Omar Faruque, Guangming Ye, Jia Li, Qiangrong Liang, Zhijie Chang, Wannian Yang, Xiao Han,
and Yuguang Shi (2012) Synapses of Amphids Defective (SAD-A) Kinase Promotes Glucose-stimulated Insulin
Secretion through Activation of p21-activated Kinase (PAK1) in Pancreatic β-Cells. J. Biol. Chem. 287:26435-
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