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Phospho-Thr402 PAK-1,2,3 Antibody

Affinity purified rabbit polyclonal antibody

  • WB - Phospho-Thr402 PAK-1,2,3 Antibody AN1080
    Western blot of rat hippocampal lysate showing specific immunolabeling of the ~68k to ~70k PAK protein (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: λ-Ptase). The blot is identical to the control except that it was incubated in λ-Ptase (1200 units for 30 min) before being exposed to the phospho-Thr402 PAK-1,2,3 antibody. The immunolabeling of PAK is completely eliminated by treatment with λ-Ptase.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P35465
Reactivity Rat
Predicted Bovine, Human, Mouse
Host Rabbit
Clonality polyclonal
Calculated MW 68/70 KDa
Additional Information
Gene ID 29431
Gene Name PAK1-3
Other Names Serine/threonine-protein kinase PAK 1, Alpha-PAK, Protein kinase MUK2, p21-activated kinase 1, PAK-1, p68-PAK, Pak1
Target/Specificity Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr402 conjugated to KLH.
Dilution WB~~ 1:1000
Format Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.
Antibody Specificity Specific for the ~68k to ~70k PAK protein phosphorylated at Thr402. Theimmunolabeling of PAK is completely eliminated by λ-phosphatase treatment.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPhospho-Thr402 PAK-1,2,3 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
ShippingBlue Ice
Research Areas
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In mammals, there are several identified isoforms of p21-activated protein kinases or PAKs: α-PAK (also known as PAK-1) and β-PAK (also known as PAK-3) are mostly brain-specific, while -PAK (also known as PAK-2) is expressed ubiquitously (Jakobi et al., 2003). Mutations of the gene coding for PAK-3 are associated with X-linked mental retardation and recent work indicates that PAK-3 is a key regulator of synapse formation and plasticity in the hippocampus (Boda et al., 2004). PAK-3 is thought to play a key role in regulation of cell shape and motility as well as cell death (Jakobi et al., 2003; Walter et al., 1998). Autophosphorylation of Thr402 in the protein has been found to be essential for activation of PAK (Jakobi et al., 2000).


Boda B, Alberi S, Nikonenko I, Node-Langlois R, Jourdain P, Moosmayer M, Parisi-Jourdain L, Muller D (2004) The
mental retardation protein PAK-3 contributes to synapse formation and plasticity in hippocampus. J Neurosci
Jakobi R, Huang Z, Walter BN, Tuazon PT, Traugh JA (2000) Substrates enhance autophosphorylation and
activation of p21-activated protein kinase gamma-PAK in the absence of activation loop phosphorylation. Eur J
Biochem 267:4414-4421.
Jakobi R, McCarthy CC, Koeppel MA, Stringer DK (2003) Caspase-activated PAK-2 is regulated by subcellular
targeting and proteasomal degradation. J Biol Chem 278:38675-38685.
Walter BN, Huang Z, Jakobi R, Tuazon PT, Alnemri ES, Litwack G, Traugh JA (1998) Cleavage and activation of
p21-activated protein kinase gamma-PAK by CPP32 (caspase 3). Effects of autophosphorylation on activity. J
Biol Chem 273:28733-28739.
Jia Nie, Chao Sun, Omar Faruque, Guangming Ye, Jia Li, Qiangrong Liang, Zhijie Chang, Wannian Yang, Xiao Han,
and Yuguang Shi (2012) Synapses of Amphids Defective (SAD-A) Kinase Promotes Glucose-stimulated Insulin
Secretion through Activation of p21-activated Kinase (PAK1) in Pancreatic β-Cells. J. Biol. Chem. 287:26435-

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$ 335.00
Cat# AN1080
(40 western blots)
Availability: 7-10 days
Bulk Size
Seasonal Special on Bulk Order
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