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Phospho-Ser301 Raf-1 Antibody

Affinity purified rabbit polyclonal antibody

  • WB - Phospho-Ser301 Raf-1 Antibody AN1084
    Western blot of UV treated human Jurkat cell lysate showing specific immunolabeling of the~74k Raf-1 protein (Control). The phosphospecificity of this labeling is shown in the second lane(lambda-phosphatase: λ-Ptase). The blot is identical to the control except that it was incubatedin λ-Ptase (1200 units for 30 min) before being exposed to the phospho Ser301 Raf-1 antibody.The immunolabeling of Raf-1 is completely eliminated by treatment with λ-Ptase.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P11345
Reactivity Human, Rat
Predicted Bovine, Chicken, Mouse, Monkey, Xenopus
Host Rabbit
Clonality polyclonal
Calculated MW 74 KDa
Additional Information
Gene ID 24703
Gene Name RAF1
Other Names RAF proto-oncogene serine/threonine-protein kinase, Proto-oncogene c-RAF, cRaf, Raf-1, Raf1, Raf
Target/Specificity Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser301 conjugated to KLH.
Dilution WB~~ 1:1000
Format Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and non-phosphopeptide affinity columns.
Antibody Specificity Specific for the ~74k Raf-1 protein phosphorylated at Ser301.Immunolabeling of the Raf-1 protein band is blocked byλ-phosphatase treatment.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPhospho-Ser301 Raf-1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
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The Ras pathway is a critical si gnal transduction cascade involved in regulating cellular proliferation, differentiation, su rvival, and oncogenic transformation. Members of the Raf serine/threonine kinase family are key intermediates in this cascade, functioning to relay signals from activated Ras to the downstream protein kinases M EK and ERK (Marshall, 1996). Previous studies have shown that phosphorylation is required for Raf-1 ac tivation (Dhillon and Kolch, 2002; Chong et al., 2003). Recent work has demonstrated that phosphorylation al so regulates the downregulation of Raf (Dougherty et al., 2005) with two sites participating: Ser 301 and Ser 642 .


Chong H, Vikis HG, Guan KL (2003) Mechanisms of regulati
ng the Raf kinase family. Cellular Signalling 15:463-469.
Dhillon AS, Kolch W (2002) Untying the regulation of
the Raf-1 kinase. Arch Biochem Biophys 404:3-9.
Dougherty MK, Muller J, Ritt DA, Zhou M, Zhou XZ, Copeland TD, Conrads TP, Veenstra TD, Lu KP, Morrison DK
(2005) Regulation of Raf-1 by Direct Feed
back Phosphorylation. Mol Cell 17:215-224.
Marshall CJ (1996) Ras effectors. current
opinion in cell biology 8:197-204.

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$ 335.00
Cat# AN1084
(40 western blots)
Availability: 7-10 days
Bulk Size
Seasonal Special on Bulk Order
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