|Predicted||Bovine, Chicken, Mouse, Monkey, Xenopus|
|Calculated MW||74 KDa|
|Other Names||RAF proto-oncogene serine/threonine-protein kinase, Proto-oncogene c-RAF, cRaf, Raf-1, Raf1, Raf|
|Target/Specificity||Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser301 conjugated to KLH.|
|Format||Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and non-phosphopeptide affinity columns.|
|Antibody Specificity||Specific for the ~74k Raf-1 protein phosphorylated at Ser301.Immunolabeling of the Raf-1 protein band is blocked byλ-phosphatase treatment.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Phospho-Ser301 Raf-1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
The Ras pathway is a critical si gnal transduction cascade involved in regulating cellular proliferation, differentiation, su rvival, and oncogenic transformation. Members of the Raf serine/threonine kinase family are key intermediates in this cascade, functioning to relay signals from activated Ras to the downstream protein kinases M EK and ERK (Marshall, 1996). Previous studies have shown that phosphorylation is required for Raf-1 ac tivation (Dhillon and Kolch, 2002; Chong et al., 2003). Recent work has demonstrated that phosphorylation al so regulates the downregulation of Raf (Dougherty et al., 2005) with two sites participating: Ser 301 and Ser 642 .
Chong H, Vikis HG, Guan KL (2003) Mechanisms of regulati
ng the Raf kinase family. Cellular Signalling 15:463-469.
Dhillon AS, Kolch W (2002) Untying the regulation of
the Raf-1 kinase. Arch Biochem Biophys 404:3-9.
Dougherty MK, Muller J, Ritt DA, Zhou M, Zhou XZ, Copeland TD, Conrads TP, Veenstra TD, Lu KP, Morrison DK
(2005) Regulation of Raf-1 by Direct Feed
back Phosphorylation. Mol Cell 17:215-224.
Marshall CJ (1996) Ras effectors. current
opinion in cell biology 8:197-204.
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