|Predicted||Bovine, Chicken, Mouse, Monkey, Xenopus|
|Calculated MW||74 KDa|
|Other Names||RAF proto-oncogene serine/threonine-protein kinase, Proto-oncogene c-RAF, cRaf, Raf-1, Raf1, Raf|
|Target/Specificity||Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser301 conjugated to KLH.|
|Format||Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and non-phosphopeptide affinity columns.|
|Antibody Specificity||Specific for the ~74k Raf-1 protein phosphorylated at Ser301.Immunolabeling of the Raf-1 protein band is blocked byλ-phosphatase treatment.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Phospho-Ser301 Raf-1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
The Ras pathway is a critical si gnal transduction cascade involved in regulating cellular proliferation, differentiation, su rvival, and oncogenic transformation. Members of the Raf serine/threonine kinase family are key intermediates in this cascade, functioning to relay signals from activated Ras to the downstream protein kinases M EK and ERK (Marshall, 1996). Previous studies have shown that phosphorylation is required for Raf-1 ac tivation (Dhillon and Kolch, 2002; Chong et al., 2003). Recent work has demonstrated that phosphorylation al so regulates the downregulation of Raf (Dougherty et al., 2005) with two sites participating: Ser 301 and Ser 642 .
Chong H, Vikis HG, Guan KL (2003) Mechanisms of regulati
ng the Raf kinase family. Cellular Signalling 15:463-469.
Dhillon AS, Kolch W (2002) Untying the regulation of
the Raf-1 kinase. Arch Biochem Biophys 404:3-9.
Dougherty MK, Muller J, Ritt DA, Zhou M, Zhou XZ, Copeland TD, Conrads TP, Veenstra TD, Lu KP, Morrison DK
(2005) Regulation of Raf-1 by Direct Feed
back Phosphorylation. Mol Cell 17:215-224.
Marshall CJ (1996) Ras effectors. current
opinion in cell biology 8:197-204.
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