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Phospho-Tyr298 EphrinB Antibody

Affinity purified rabbit polyclonal antibody

  • WB - Phospho-Tyr298 EphrinB Antibody AN1088
    Western blot of rat testes lysate showing specific immunolabeling of the ~46k EphrinB phosphorylated at Tyr298 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: λ-Ptase). The blot is identical to the control except that it was incubated in λ-Ptase (1200 units for 30 min) before being exposed to the Anti-Tyr298 EphrinB. The immunolabeling of the EphrinB band is completely eliminated by treatment with λ-Ptase.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P28693
Reactivity Rat
Predicted Bovine, Chicken, Human, Mouse, Monkey, Xenopus, Zebrafish
Host Rabbit
Clonality polyclonal
Calculated MW 46 KDa
Additional Information
Gene ID 396513
Gene Name EPHB2
Other Names Ephrin type-B receptor 2, EPH-like kinase 5, EK5, cEK5, EPHB2, CEK5
Target/Specificity Synthetic phospho-peptide corresponding to amino acid residues surrounding Tyr298 conjugated to KLH.
Dilution WB~~ 1:1000
Format Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.
Antibody Specificity Specific for the ~46k EphrinB protein phosphorylated at Tyr298.Immunolabeling of the EphrinB band is blocked by λ-phosphatase treatment.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPhospho-Tyr298 EphrinB Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
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Research Areas
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EphrinB proteins are thought to play key roles in cellular functions as diverse as neuronal migration and blood vessel development (Flanagan and Vancerhaeghen, 1998; Dufour et al., 2003; Oike et al., 2002). EphrinB molecules expressed at the membrane surface bind to the EphB family receptors on target cells during cell-to cell contact. This interaction leads to cell signaling in the target cell but also generates a reverse signal in the cell expressing EphrinB on its surface. This reverse signaling event is thought to be critical for vessel maturation and neuronal development. Importantly, tyrosine phosphorylation of EphrinB is thought to be a critical component of this reverse signaling event (Palmer et al., 2002). Recent work suggests that phosphorylation of a specific EphrinB residue (Tyr298) plays a key role in EphrinB signaling (Kalo, et al., 2001).


Bong, Y.S., Park, Y.H., Lee, H.S., Mood, K., Ishimura, A. and Daar, I.O. Tyr-298 in ephrinB1 is critical for an
interaction with the Grb4 adaptor protein, Biochem. J. 377:499-507 (2004).
Dufour, A., Seibt, J., Passante, L., Depaepe, V., Ciossek, T., Frisen, J., Kullander, K., Flanagan, J.G., Polleux, F. and
Vanderhaeghen, P. Area specificity and topography of thalamocortical projections are controlled by ephrin/Eph
genes, Neuron 39:453-465 (2003).
Flanagan, J.G. and Vanderhaeghen, P. The ephrins and Eph receptors in neural development, Annu. Rev. Neurosci.
21:309-345 (1998).
Oike, Y., Ito, Y., Hamada, K., Zhang, X.Q., Miyata, K., Arai, F., Inada, T., Araki, K., Nakagata, N., Takeya, M.,
Kisanuki, Y.Y., Yanagisawa, M., Gale, N.W. and Suda, T, Regulation of vasculogenesis and angiogenesis by
EphB/ephrin-B2 signaling between endothelial cells and surrounding mesenchymal cells, Blood 100:1326-1333
Palmer, A., Zimmer, M., Erdmann, K.S., Eulenburg, V., Porthin, A., Heumann, R., Deutsch, U. and Klein, R Ephrin B
phosphorylation and reverse signaling: regulation by Src kinases and PTP-BL Phosphatase, Mol Cell 9:725-737

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$ 335.00
Cat# AN1088
(40 western blots)
Availability: 7-10 days
Bulk Size
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