|Predicted||Bovine, Chicken, Human, Mouse, Monkey, Xenopus, Zebrafish|
|Calculated MW||46 KDa|
|Other Names||Ephrin type-B receptor 2, EPH-like kinase 5, EK5, cEK5, EPHB2, CEK5|
|Target/Specificity||Synthetic phospho-peptide corresponding to amino acid residues surrounding Tyr298 conjugated to KLH.|
|Format||Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.|
|Antibody Specificity||Specific for the ~46k EphrinB protein phosphorylated at Tyr298.Immunolabeling of the EphrinB band is blocked by λ-phosphatase treatment.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Phospho-Tyr298 EphrinB Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
EphrinB proteins are thought to play key roles in cellular functions as diverse as neuronal migration and blood vessel development (Flanagan and Vancerhaeghen, 1998; Dufour et al., 2003; Oike et al., 2002). EphrinB molecules expressed at the membrane surface bind to the EphB family receptors on target cells during cell-to cell contact. This interaction leads to cell signaling in the target cell but also generates a reverse signal in the cell expressing EphrinB on its surface. This reverse signaling event is thought to be critical for vessel maturation and neuronal development. Importantly, tyrosine phosphorylation of EphrinB is thought to be a critical component of this reverse signaling event (Palmer et al., 2002). Recent work suggests that phosphorylation of a specific EphrinB residue (Tyr298) plays a key role in EphrinB signaling (Kalo, et al., 2001).
Bong, Y.S., Park, Y.H., Lee, H.S., Mood, K., Ishimura, A. and Daar, I.O. Tyr-298 in ephrinB1 is critical for an
interaction with the Grb4 adaptor protein, Biochem. J. 377:499-507 (2004).
Dufour, A., Seibt, J., Passante, L., Depaepe, V., Ciossek, T., Frisen, J., Kullander, K., Flanagan, J.G., Polleux, F. and
Vanderhaeghen, P. Area specificity and topography of thalamocortical projections are controlled by ephrin/Eph
genes, Neuron 39:453-465 (2003).
Flanagan, J.G. and Vanderhaeghen, P. The ephrins and Eph receptors in neural development, Annu. Rev. Neurosci.
Oike, Y., Ito, Y., Hamada, K., Zhang, X.Q., Miyata, K., Arai, F., Inada, T., Araki, K., Nakagata, N., Takeya, M.,
Kisanuki, Y.Y., Yanagisawa, M., Gale, N.W. and Suda, T, Regulation of vasculogenesis and angiogenesis by
EphB/ephrin-B2 signaling between endothelial cells and surrounding mesenchymal cells, Blood 100:1326-1333
Palmer, A., Zimmer, M., Erdmann, K.S., Eulenburg, V., Porthin, A., Heumann, R., Deutsch, U. and Klein, R Ephrin B
phosphorylation and reverse signaling: regulation by Src kinases and PTP-BL Phosphatase, Mol Cell 9:725-737
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