|Predicted||Bovine, Mouse, Monkey, Xenopus, Zebrafish|
|Calculated MW||66 KDa|
|Other Names||Serine/threonine-protein kinase PLK1, Polo-like kinase 1, PLK-1, Serine/threonine-protein kinase 13, STPK13, PLK1, PLK|
|Target/Specificity||Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr210 conjugated to KLH.|
|Format||Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.|
|Antibody Specificity||Specific for ~66k PLK phosphorylated at Thr210. Immunolabeling of the PLK band is completely blocked by λ-phosphatase treatment.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Phospho-Thr210 Polo-like Kinase 1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
Polo-like kinases are important regulators of cell cycle progression. PLK1 is a highly conserved Ser/Thr kinase that has essential roles in the formation of mitotic bipolar spindles (van Vugt et al., 2004). Deregulated expression of PLK’s is detected in many types of cancer and associated with oncogenesis (Takei et al., 2005). It has been proposed that PLK1 function is altered at different stages of mitosis through consecutive phosphorylation events at Ser137 and Thr210 (van de Weerdt et al., 2005).
van Vugt MA, van de Weerdt BC, Vader G, Janssen H, Calafat J, Klompmaker R, Wolthuis RM, Medema RH (2004) Polo-like kinase 1 is required for bipolar spindle formation but is dispesible for anaphase promoting complex cdc20 activation and initiation of cytokinesis. J. Biol. Chem. Aug 27;279(35):36841-54.
Takai N, Hamanaka R, Yoshimatsu J, Miyakawa I (2005) Polo-like kinases and cancer. Oncogene Jan 10; 24(2): 287-91.
van de Weerdt BC, van Vugt MA, Lindon C, Kauw JJ, Rozendaal MJ, Klompmaker R, Wolthuis RM, Medema RH (2005) Uncoupling anaphase-promoting complex/cyclosome activity from spindle assembly checkpoint control by deregulating polo-like kinase 1. Mol. Cell Biol. Mar; 25(5):2031-44.
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