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Glial Fibrillary Acidic Protein (GFAP) Antibody

Rabbit polyclonal antibody

     
  • WB - Glial Fibrillary Acidic Protein (GFAP) Antibody AN1143
    Western blot of rat cortex lysate showing specific immunolabeling of the ~ 50k GFAP protein.
    detail
  • IF - Glial Fibrillary Acidic Protein (GFAP) Antibody AN1143
    Immunofluorescence showing specific labeling of GFAP (red) and vimentin (green) in cultured neurons and glia. Cells containing GFAP and vimentin appear yellow.
    detail
  • SPECIFICATION
  • CITATIONS: 1
  • PROTOCOLS
  • BACKGROUND
  • detail
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IF
Primary Accession Q28115
Reactivity Rat
Predicted Human, Mouse
Host rabbit
Clonality polyclonal
Calculated MW 50 KDa
Additional Information
Gene ID 281189
Gene Name GFAP
Other Names Glial fibrillary acidic protein, GFAP, GFAP
Target/Specificity Recombinant and purified bovine GFAP.
Dilution WB~~ 1:5000
IF~~ 1:1000
Format Unpurified neat serum
Antibody Specificity Specific for the ~50kDa GFAP protein. A lower band at ~45kDa is a proteolytic fragment derived from the GFAP molecule.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGlial Fibrillary Acidic Protein (GFAP) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
ShippingBlue Ice
Research Areas
Citations ( 0 )

Background

Glial Fibrillary Acidic Protein (GFAP) was discovered by Amico Bignami and co-workers as a major fibrous protein of multiple sclerosis plaques (1). It was subsequently found to be a member of the 10nm or intermediate filament (IF) family, specifically the IF family Class III, which also includes peripherin, desmin and vimentin. GFAP is strongly and specifically expressed in astrocytes and certain other astroglia in the CNS, in satellite cells, peripheral ganglia, and in non-myelinating Schwann cells in peripheral nerves. In many damage and disease states GFAP expression is heavily upregulated in astrocytes. In addition, neural stem cells frequently strongly express GFAP. Point mutations in the protein coding region of the GFAP gene lead to Alexander disease which is characterized by the presence of abnormal astrocytes containing GFAP protein aggregates known as Rosenthal fibers (2).

References

1. Bignami A, Eng LF, Dahl D, Uyeda CT. Localization of the glial fibrillary acidic protein in astrocytes by immunofluorescence. Brain Res. 43:429-35 (1972).
2. Brenner M, Johnson AB, Boespflug-Tanguy O, Rodriguez D, Goldman JE and Messing A. Mutations in GFAP, encoding glial fibrillary acidic protein, are associated with Alexander disease. Nat Genet 27:117-20 (2001)

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$ 310.00
Cat# AN1143
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Availability: 7-10 days
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