|Application ||IHC, IF, WB, FC|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||28 KDa|
|Other Names||Cyclin-dependent-like kinase 5, Cell division protein kinase 5, Serine/threonine-protein kinase PSSALRE, Tau protein kinase II catalytic subunit, TPKII catalytic subunit, Cdk5, Cdkn5|
|Target/Specificity||Purified rat Cdk5.|
|Format||Protein G purified culture supernatant.|
|Antibody Specificity||The antibody is specific for the ~ 28kDa Cdk5 protein in Western blots of rat striatal lysate. The antibody shows no cross reactivity with the Cdk5 cofactor p35 or its degradation product p25. This monoclonal also works well for immunocytochemistry using primary cultured rat neurons and for immunohistochemistry on mouse brain tissue.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Cdk5 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
The neuronal protein kinase, Cdk5 has been implicated in a vast array of normal neuronal functions including regulation of neurotransmitter synthesis (Kansy J et al., 2004), the presynaptic vesicle cycle (Nguyen, C. & Bibb, JA 2003), neurotransmitter receptor trafficking and dopamine neurotransmission (Bibb, JA et al. 1999). At the same time Cdk5 has been implicated in a plethora of neurological and neuropsychiatric disorders including Alzheimer’s, Parkinson’s, Huntington’s, epilepsy, schizophrenia, and drug addiction. Detection of Cdk5 in normal samples as well as tissue undergoing neurodegeneration may advance studies in these areas. Moreover, this antibody may allow more accurate postmortem evaluations of Cdk5 protein expression, and thus serve as a valuable new reagent for neuropathology.
Kansy, J. et al. Identification of tyrosine hydroxylase as a physiological substrate of Cdk5. J. Neurochem. 91, 374-384 (2004).
Nguyen, C. & Bibb, J. A. Cdk5 and the mystery of synaptic vesicle endocytosis. J Cell Biol. 163, 697- 699 (2003).
Bibb, J. A. et al. Phosphorylation of DARPP-32 by Cdk5 modulates dopamine signaling in neurons. Nature 402, 669-671 (1999).
Lagace, D. C. et al. Cdk5 is essential for adult hippocampal neurogenesis. Proc Natl Acad Sci USA 105, 18567–18571 (2008).
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