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Park7 (DJ-1) Antibody

Mouse monoclonal antibody

  • FC - Park7 (DJ-1) Antibody AN1214
    Flow cytometric analysis of Hela cells using Park7 (DJ-1) Antibody(green, Cat#AN1214) compared to an isotype control of mouse IgG1(blue). AN1214 was diluted at 1:500 dilution. An Alexa Fluor® 488 goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody.
  • WB - Park7 (DJ-1) Antibody AN1214
    Western blot of rat kidney lysate showing specific immunolabeling of the ~21k park7 protein.
  • IF - Park7 (DJ-1) Antibody AN1214
    Immunofluorescence of HeLa cells stained with anti-park7 (green) showing strong cytoplasmic staining and anti-vimentin antibody (red).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O88767
Reactivity Bovine, Human, Mouse, Rat
Host Mouse
Clonality monoclonal
Isotype IgG1
Clone Names 4H4
Calculated MW 21 KDa
Additional Information
Gene ID 117287
Gene Name PARK7
Other Names Protein DJ-1, 34--, Contraception-associated protein 1, Protein CAP1, Fertility protein SP22, Parkinson disease protein 7 homolog, Park7, Cap1
Target/Specificity Full length recombinant human Park7 expressed in and purified from E. coli.
Dilution FC~~1:500
WB~~ 1:2000
IF~~ 1:500
Format Affinity purified from tissue culture supernatant
Antibody Specificity Specific for the ~21k park7 protein
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPark7 (DJ-1) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
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Research Areas
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Park7, also known as DJ-1, is a member of the peptidase C56 family of proteins and is thought to function as a molecular chaperone. Mutations in park7 have been associated with autosomal recessive, early onset Parkinson’s disease (Bonifati et al., 2003). Recently, park7 has been shown to inhibit microtubule associated protein 1B aggregation thus leading to neuronal apoptosis (Wang et al., 2011).


Bonifati V, Rizzu P, van Baren MJ, Schaap O, Breedveld GJ, Krieger E, Dekker MC, Squitieri F, Ibanez P, Joosse M, van Dongen JW, Vanacore N, van Swieten JC, Brice A, Meco G, van Duijn CM, Oostra BA, Heutink P. (2003) Mutations in the DJ-1 gene associated with autosomal recessive early-onset parkinsonism. Science. Jan 10;299(5604):256-9
Wang Z, Zhang Y, Zhang S, Guo Q, Tan Y, Wang X, Xiong R, Ding J, Chen S. (2011) DJ-1 can inhibit microtubule associated protein 1 B formed aggregates. Mol Neurodegener. Jun 6;6:38

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$ 335.00
Cat# AN1214
(40 western blots)
Availability: 7-10 days
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