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>   home   >   Products   >   Primary Antibodies   >   Antibody Collections   >   Phospho Antibodies   >   Phospho-Ser499 FMRP (Fragile X Mental Retardation Protein) Antibody   

Phospho-Ser499 FMRP (Fragile X Mental Retardation Protein) Antibody

Affinity purified rabbit polyclonal antibody

  • WB - Phospho-Ser499 FMRP (Fragile X Mental Retardation Protein) Antibody AN1228
    Western blot of rat hippocampal lysate showing specific immunolabeling of the ~71k FMRPprotein phosphorylated at Ser499 (Control). The phosphospecificity of this labeling is shown inthe second lane (lambda-phosphatase: λ-Ptase). The blot is identical to the control exceptthat the lysate was incubated in λ-Ptase (400 units/100ul lysate for 30 min) before being exposedto the FMRP Ser499 antibody. The immunolabeling is completely eliminated by treatment with λ-Ptase.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q80WE1
Reactivity Rat
Predicted Bovine, Chicken, Human, Mouse, Monkey, Xenopus, Zebrafish
Host Rabbit
Clonality polyclonal
Calculated MW 71 KDa
Additional Information
Gene ID 24948
Gene Name FMR1
Other Names Fragile X mental retardation protein 1 homolog, FMRP, Protein FMR-1, Fmr1
Target/Specificity Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser499 conjugated to KLH.
Dilution WB~~ 1:1000
Format Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.
Antibody Specificity Specific for ~71k FMRP protein phosphorylated at Ser499. Immunolabeling of the FMRP protein is completely eliminated bylambda-phosphatase.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPhospho-Ser499 FMRP (Fragile X Mental Retardation Protein) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
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Research Areas
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Fragile X Mental Retardation Protein (FMRP) is an RNA-binding protein that plays an essential role in cognitive brain function. Mutations in the FMR1 gene, which codes for FMRP, can result in fragile X syndrome, autism, as well as other cognitive deficits (Brown et al.,1998, Goodlin-Jones et al., 2004). Phosphorylation of the highly conserved Ser499 has been shown to trigger hierarchical phosphorylation of nearby serines and may play a role in suppressing target mRNA translation (Ceman et al., 2003, Narayanan et al. 2008).


Bernard PB, Castano AM, O'Leary H, Simpson K, Browning MD, Benke TA. (2013) Phosphorylation of FMRP and alterations of FMRP complex underlie enhanced mLTD in adult rats triggered by early life seizures. Neurobiol Dis. Nov; 59:1-17.
Brown V, Small K, Lakkis L, Feng Y, Gunter C, Wilkinson KD, Warren ST (1998) Purified recombinant Fmrp exhibits selective RNA binding as an intrinsic property of the fragile X mental retardation protein. J Biol Chem 273:15521–15527
Ceman S, O'Donnell WT, Reed M, Patton S, Pohl J, Warren ST. (2003) Phosphorylation influences the translation state of FMRP-associated polyribosomes. Hum Mol Genet. Dec 15;12(24):3295-305
Goodlin-Jones BL, Tassone F, Gane LW, Hagerman RJ. (2004) Autistic spectrum disorder and the fragile X premutation. J Dev Behav Pediatr. Dec;25(6):392-8
Narayanan U, Nalavadi V, Nakamoto M, Thomas G, Ceman S, Bassell GJ, Warren ST. (2008) S6K1 phosphorylates and regulates fragile X mental retardation protein (FMRP) with the neuronal protein synthesis-dependent mammalian target of rapamycin (mTOR) signaling cascade. J Biol Chem 283:18478–18482

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$ 335.00
Cat# AN1228
(40 western blots)
Availability: 7-10 days
Bulk Size
Seasonal Special on Bulk Order
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