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>   home   >   Products   >   Primary Antibodies   >   Antibody Collections   >   Phospho Antibodies   >   Phospho-Ser358 Eukaryotic Elongation Factor 1 alpha 2 (eEF1A2) Antibody   

Phospho-Ser358 Eukaryotic Elongation Factor 1 alpha 2 (eEF1A2) Antibody

Affinity purified rabbit polyclonal antibody

  • WB - Phospho-Ser358 Eukaryotic Elongation Factor 1 alpha 2 (eEF1A2) Antibody AN1251
    Western blot of mouse hippocampus lysate showing specific immunolabeling of the ~ 50k eEF1A2 protein phosphorylated at Ser358. Immunolabeling is blocked by treatment of the lysate with lambda phosphatase (P-tase).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q05639
Reactivity Mouse
Predicted Chicken, Human, Monkey, Rat, Xenopus
Host Rabbit
Clonality polyclonal
Calculated MW 50 KDa
Additional Information
Gene ID 1917
Gene Name EEF1A2
Other Names Elongation factor 1-alpha 2, EF-1-alpha-2, Eukaryotic elongation factor 1 A-2, eEF1A-2, Statin-S1, EEF1A2, EEF1AL, STN
Target/Specificity Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser358 conjugated to KLH.
Dilution WB~~ 1:1000
Format Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.
Antibody Specificity Specific for the ~50k eEF1A2 phosphorylated at Ser358. Immunolabeling of the eEF1A2 band is blocked by the treatment of the lysate with lambda phosphatase (30 minutes, 800 units / 1 mg protein).
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPhospho-Ser358 Eukaryotic Elongation Factor 1 alpha 2 (eEF1A2) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
ShippingBlue Ice
Research Areas
Citations ( 0 )


Eukaryotic Elongation Factor eEF1A exists in two variant forms, eEF1A1 and eEF1A2. While eEF1A1 is almost ubiquitously expressed in humans, eEF1A2 is predominantly found in heart, brain, and skeletal muscle (Knudsen et al., 1993). Expression of eEF1A2 may have a role in ovarian cancer, as its expression is drastically increased in human ovarian tumors (Anand et al., 2002). Due to differences in structural models between the two isoforms, eEF1A1 and eEF1A2 likely have variant-specific phosphorylation sites (Soares et al., 2009). Ribosome-associated JNK phosphorylates Ser358 on eEF1A2 to promote degradation of newly synthesized polypeptides by the proteasome (Gandin et al., 2013).


Knudsen SM, Frydenberg J, Clark BF, Leffers H. Tissue-dependent variation in the expression of elongation factor-1 alpha isoforms: isolation and characterization of a cDNA encoding a novel variant of human elongation-factor 1 alpha. Eur J Biochem. 1993;215:549-554.
Anand N, Murphy s, Amann G, Wernick M, Porter LA, Cukier IH, Collins C, Gray JW, Diebold J, Demetrick DJ. Protein elongation factor EEF1A2 is a putative oncogene in ovarian cancer. Nat Genet. 2002;31:301-305.
Soares DC, Barlow PN, Newbery HJ, Porteous DJ, Abbot CM. Structural models of human eEF1A1 and eEF1A2 reveal two distant surface clusters of sequence variation and potential differences in phosphorylation. PLoS One. 2009 Jul 28;4(7):e6315.
Gandin V, Gutierrez GJ, Brill LM, Varsano T, Feng Y, Aza-Blanc P, Au Q, McLaughlan S, Ferreira TA, Alain T, Sonenberg N, Topisirovic I, Ronai ZA. Degradation of newly synthesized polypeptides by ribosome-associated RACK1/c-Jun N-Terminal Kinase/Eukaryotic Elongation Factor 1A2 complex. 2013 Jul;33(13):2510-26.

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$ 335.00
Cat# AN1251
(40 western blots)
Availability: 7-10 days
Bulk Size
Seasonal Special on Bulk Order
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