Foundational characteristics of cancer include proliferation, angiogenesis, migration, evasion of apoptosis, and cellular immortality. Find key markers for these cellular processes and antibodies to detect them.
The SUMOplot™ Analysis Program predicts and scores sumoylation sites in your protein. SUMOylation is a post-translational modification involved in various cellular processes, such as nuclear-cytosolic transport, transcriptional regulation, apoptosis, protein stability, response to stress, and progression through the cell cycle.
The Autophagy Receptor Motif Plotter predicts and scores autophagy receptor binding sites in your protein. Identifying proteins connected to this pathway is critical to understanding the role of autophagy in physiological as well as pathological processes such as development, differentiation, neurodegenerative diseases, stress, infection, and cancer.
Phospho-Ser244 Ribosomal Protein S6 Antibody
Affinity purified rabbit polyclonal antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB |
|---|---|
| Primary Accession | P62754 |
| Reactivity | Human, Mouse |
| Predicted | Chicken, Pig, Monkey, Rat, Xenopus, Zebrafish |
| Host | Rabbit |
| Clonality | polyclonal |
| Calculated MW | 28 KDa |
| Gene ID | 20104 |
|---|---|
| Gene Name | RPS6 |
| Other Names | 40S ribosomal protein S6, Phosphoprotein NP33, Rps6 |
| Target/Specificity | Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser244 conjugated to KLH. |
| Dilution | WB~~ 1:1000 |
| Format | Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns. |
| Antibody Specificity | Specific for the ~28k rpS6 protein phosphorylated at Ser244 |
| Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | Phospho-Ser244 Ribosomal Protein S6 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Shipping | Blue Ice |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
info@abcepta.com, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
Background
Ribosomal protein S6 (rpS6) is a crit ical component of the 40 S ribosomal subunit that mediates translation initiation at the 5 ′ -m 7 GpppG cap of mRNA. The rpS6 protein is both cytoplasmic and nuclear localized (Chen and Dittmer 2011). In response to mitogenic stimuli, rpS6 undergoes ordered C-terminal phosphorylation by p70 S6 kinases and p90 ribosomal S6 kinases on four Ser residues (Ser-235, Ser-236, Ser-240, and Ser-244) whose modification potentiates rpS6 cap binding activity (Hutchinson et al., 2011). Additionally, rpS6 phosphorylation and function are highly regulated and have been implicated in the regulation of translational initiation and protein synthesis in response to extracellular stimuli such as TRAIL and gamma interferon (IFN- γ ), as well as upon activation of the phosphatidylinositol 3-kinase (PI3K)–A kt–mTOR pathway (Chen and Dittmer 2011).
References
Chen W and Dittmer D (2011) Ribosomal protein S6 (RPS6) interacts with the latency-associated nuclear
antigen (LANA) of Kaposi sarcoma associated
herpesvirus (KSHV). J. Virol. 18 9495-9505
Fumagalli S. and Thomas, G. (2000). S6 phosphorylation
and signal transduction. In Translational control
of gene expression (eds. N. Sonenberg et al.), pp.
695-717. Cold Spring Harbor Laboratory Press,
Cold Spring Harbor, NY.
Hutchinson JA, Shanware NP, Chang H, Tibbetts RS
. (2011
) Regulation of ribosomal protein S6
phosphorylation by casein kinase 1 and prot
ein phosphatase 1. J Biol Chem 10:8688-96
If you have used an Abcepta product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at tech@abcepta.com.
Ordering Information
Other Products
Shipping Information
