|Application ||WB, IHC, E|
|Description||Dynamin-1 (Dyn1), with 864-amino acid protein (about 95kDa), belongs to the dynamin family. Dynamin-1 (neuron-specific), dynamin-2 (ubiquitously expressed), and dynamin-3 (expressed only in the testis, brain, and lung), constitute the dynamin family. Members of the dynamin family are GPTase, microtubule-associated proteins which are involved in endocytosis, synaptic transmission and neurogenesis. Dynamin-1 is phosphorylated in nerve terminals exclusively in the cytosolic compartment and in vitro by protein kinase C. Dynamin-1 is a large GTPase enzyme required in membrane constriction and fission during multiple forms of endocytosis. Dynamin-1 is also a key molecule required for the recycling of synaptic vesicles in neurons, and it has been known that dynamin-1 gene expression is induced during neuronal differentiation.|
|Immunogen||Purified recombinant fragment of human Dynamin-1 expressed in E. Coli.|
|Formulation||Ascitic fluid containing 0.03% sodium azide.|
|Other Names||Dynamin-1, 188.8.131.52, DNM1, DNM|
|Dilution||WB~~1/500 - 1/2000|
IHC~~1/200 - 1/1000
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Dynamin-1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Microtubule-associated force-producing protein involved in producing microtubule bundles and able to bind and hydrolyze GTP. Most probably involved in vesicular trafficking processes. Involved in receptor-mediated endocytosis.|
|Cellular Location||Cytoplasm. Cytoplasm, cytoskeleton Note=Microtubule-associated|
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1. Annie Quan and Phillip J. Robinson. Methods Enzymol. 2005; 404:556-69. 2. Jiyun Yoo, Moon-Jin Jeong, Byoung-Mog Kwon. J. Biol. Chem., Mar 2002; 277: 11904 – 11909
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