|Application ||WB, IHC, E|
|Clone Names||3C10F7; 3C10G5; PMC01; 4D12B3; 3C10F7C11|
|Calculated MW||66039 Da|
|Description||CK1 (also designated Cytokeratin 1 or KRT1 ),with 644-amino acid protein (about 70kDa), is a member of the keratin family. Cytokeratins play a critical role in differentiation and tissue specialization, and they function to maintain the overall structural integrity of epithelial cells.CK1 consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. CK1 is specifically expressed in the spinous and granular layers of the epidermis with family member KRT10 and mutations in these genes have been associated with bullous congenital ichthyosiform erythroderma.|
|Immunogen||Purified recombinant fragment of CK1 expressed in E. Coli.|
|Formulation||Ascitic fluid containing 0.03% sodium azide.|
|Other Names||Keratin, type II cytoskeletal 1, 67 kDa cytokeratin, Cytokeratin-1, CK-1, Hair alpha protein, Keratin-1, K1, Type-II keratin Kb1, KRT1, KRTA|
|Dilution||WB~~1/500 - 1/2000|
IHC~~1/200 - 1/1000
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||CK1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||May regulate the activity of kinases such as PKC and SRC via binding to integrin beta-1 (ITB1) and the receptor of activated protein kinase C (RACK1/GNB2L1). In complex with C1QBP is a high affinity receptor for kininogen-1/HMWK.|
|Cellular Location||Cell membrane. Note=Located on plasma membrane of neuroblastoma NMB7 cells|
|Tissue Location||The source of this protein is neonatal foreskin. The 67-kDa type II keratins are expressed in terminally differentiating epidermis|
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1. Ricardo G. Espinola, Robin A. Pixley, Berhane Ghebrehiwet. Blood (ASH Annual Meeting Abstracts), Nov 2005; 106: 2666.
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