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Myoglobin Antibody

Purified Mouse Monoclonal Antibody

  • WB - Myoglobin Antibody AO1074a
    Figure 1: Western blot analysis using Myoglobin mouse mAb against truncated Myoglobin recombinant protein(AA: 2-154).
  • IHC - Myoglobin Antibody AO1074a
    Figure 2: Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue showing cytoplasmic localization using anti-Myoglobin antibody with DAB staining.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P02144
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 5A2G8
Isotype IgG1
Calculated MW 17184 Da
Description Myoglobin (MB), with 154-amino acid protein (about 17kDa), is a member of the globin superfamily and expression of myoglobin is highest in skeletal and cardiac muscle. Functionally, myoglobin is well accepted as an O2-storage protein in muscle, capable of releasing O2 during periods of hypoxia or anoxia. Myoglobin is also thought to buffer intracellular O2 concentration when muscle activity increases and to facilitate intracellular O2 diffusion by providing a parallel path that augments simple diffusion of dissolved O2. Furthermore, myoglobin is used together with cTnI or cTnT in clinical practise for better specificity in AMI diagnosis.
Immunogen Purified recombinant fragment of Myoglobin expressed in E. Coli.
Formulation Purified antibody in PBS containing 0.03% sodium azide.
Additional Information
Gene ID 4151
Other Names Myoglobin, MB
Dilution WB~~1/500 - 1/2000
IHC~~1/200 - 1/1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMyoglobin Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MB
Function Serves as a reserve supply of oxygen and facilitates the movement of oxygen within muscles.
Research Areas
Citations (0)

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1. George A. Ordway, Daniel J. Garry. J. Exp. Biol., Sep 2004; 207: 3441-3446. 2. Ulrich Floel, Tim Laussmann, Axel Goecke. Circ. Res., Apr 2005; 96: e68 - e75.

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$ 300.00
Cat# AO1074a
(40 western blots)
Availability: 7-10 days
Bulk Size
Seasonal Special on Bulk Order
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