- Antibodies
- New
- Biological Process >
- Cellular Compartment >
- Disease >
- Molecular Function >
- Pathway Biocarta >
- Pathway KEGG >
- Pathway Panther >
- Tissue >
- Tag
- Peptides
- Biological Process >
- Cellular Compartment >
- Disease >
- Molecular Function >
- Pathway Biocarta >
- Pathway KEGG >
- Pathway Panther >
- Tissue >
- Amino Acids
- Tag
- Biological Process >
- Proteins
- RNAi
- FL cDNA Clones
- Cell/Tissues/Lysates
MAPK1 AntibodyMouse Monoclonal Antibody to ERK2
| Country | United States
Ordering Information
Australia Austria Belgium Brazil Bulgaria Canada China Croatia Cyprus Czech Republic Denmark Estonia Finland France Germany Greece Hungary Iceland India Indonesia Ireland Israel Italy Japan Korea Latvia Lithuania Luxembourg Macedonia Malaysia Malta Netherlands Norway Pakistan Poland Portugal Romania Serbia Singapore Slovakia Slovenia Spain Sweden Switzerland Taiwan Turkey United Kingdom United States Vietnam Others 
|
|||
|---|---|---|---|---|
| Catalog # | Size | Availability | Price | |
| AO1078a | 0.1mg 400 ul | 7-10 days | $ 300.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
- Reviews
- Protocols
- Backgrounds
MAPK1 Antibody - Product info | |
| Application | WB, IHC, IF
|
| Primary Accession | P28482 |
| Other Accession | NP_620407.1 |
| Reactivity | Human, Mouse, Monkey |
| Isotype | Mouse IgG2a |
| Clone Names | 4C11 |
| Calculated MW | 41390 Da |
MAPK1 Antibody - Additional info | |
| Gene ID 5594 | |
| Other Names ERK; p38; p40; p41; ERT1; MAPK2; PRKM1; P42MAPK; p41mapk; MAPK1 | |
| Target/Specificity Purified recombinant fragment of human ERK2 expressed in E. Coli. | |
| Dilution WB~~1:500 - 2000. IHC~~1:200 - 1000. IF~~1:200 - 1000. | |
| Format Purified recombinant fragment of human ERK2 expressed in E. Coli. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions MAPK1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. | |
MAPK1 Antibody - Protein Information | |
| Name MAPK1 | |
| Synonyms ERK2, PRKM1, PRKM2 | |
| Function Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The MAPK/ERK cascade plays also a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. About 160 substrates have already been discovered for ERKs. Many of these substrates are localized in the nucleus, and seem to participate in the regulation of transcription upon stimulation. However, other substrates are found in the cytosol as well as in other cellular organelles, and those are responsible for processes such as translation, mitosis and apoptosis. Moreover, the MAPK/ERK cascade is also involved in the regulation of the endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC); as well as in the fragmentation of the Golgi apparatus during mitosis. The substrates include transcription factors (such as ATF2, BCL6, ELK1, ERF, FOS, HSF4 or SPZ1), cytoskeletal elements (such as CANX, CTTN, GJA1, MAP2, MAPT, PXN, SORBS3 or STMN1), regulators of apoptosis (such as BAD, BTG2, CASP9, DAPK1, IER3, MCL1 or PPARG), regulators of translation (such as EIF4EBP1) and a variety of other signaling-related molecules (like ARHGEF2, DCC, FRS2 or GRB10). Protein kinases (such as RAF1, RPS6KA1/RSK1, RPS6KA3/RSK2, RPS6KA2/RSK3, RPS6KA6/RSK4, SYK, MKNK1/MNK1, MKNK2/MNK2, RPS6KA5/MSK1, RPS6KA4/MSK2, MAPKAPK3 or MAPKAPK5) and phosphatases (such as DUSP1, DUSP4, DUSP6 or DUSP16) are other substrates which enable the propagation the MAPK/ERK signal to additional cytosolic and nuclear targets, thereby extending the specificity of the cascade. May play a role in the spindle assembly checkpoint | |
| Cellular Location Cytoplasm, cytoskeleton, spindle (By similarity). Nucleus. Cytoplasm, cytoskeleton, centrosome Cytoplasm. Note=Associated with the spindle during prometaphase and metaphase (By similarity). PEA15-binding and phosphorylated DAPK1 promote its cytoplasmic retention. Phosphorylation at Ser- 246 and Ser-248 as well as autophosphorylation at Thr-190 promote nuclear localization | |
MAPK1 Antibody - Related products
AP7250b: MAPK1 Antibody (C-term)
AP7250c: MAPK1 Antibody (Center)
AP7250d: MAPK1 Antibody (T184)
AP7250e: MAPK1 Antibody (T204)
AP7501a: MAPK1 Antibody (C-term)
RI13183: MAPK1 predesign siRNA
BP7250b: MAPK1 Antibody (C-term) Blocking Peptide
BP7250d: MAPK1 Antibody (T184) Blocking Peptide
BP7250e: MAPK1 Antibody (T204) Blocking Peptide
BP7501a: ERK2 Antibody (C-term) Blocking Peptide
AT2781a: MAPK1 Antibody (monoclonal) (M01)
MAPK1 Antibody - Application data
-
Figure 1: Western blot analysis using anti-ERK2 monoclonal antiobdy against Hela (1), NIH/3T3 (2), MCF-7 (3), HEK293 (4), Jurkat (5), A549 (6), NTERA-2 (7) and SMMC-7721 (8) cell lysate.
-
Figure 2: Immunohistochemical analysis of paraffin-embedded human lung carcinoma(left) and breast carcinoma(right) showing cytoplasmic localization using anti-ERK2 antibody with DAB staining.
-
Figure 3: Confocal immunofluorescence analysis of Eca-109 cells using anti-ERK2 mAb (green).
MAPK1 Antibody - Research Areas
Abgent welcomes feedback from its customers.
If you have used an Abgent product and would like to share how it has performed, please click on the
"Submit Review" button and provide the requested information. Our staff will examine and post your
review and contact you if needed.
If you have any additional inquiries please email technical services at tech@abgent.com.
Thank you for your support.
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
ERK2 (also designated extracellular-signal-related kinase 2 or mitogen-activated protein kinase 1), with 360-amino acid protein (about 40kDa), belongs to the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of ERK2 requires its phosphorylation by upstream kinases. ERK2 is located in the cytoplasm of resting cells and translocates into the nucleus upon extracellular stimuli by active transport of a dimer. ERK2 is essential for placental development and ERK2 in the trophoblast compartment may be indispensable for the vascularization of the labyrinth.
REFERENCES
1. Angelique W. Whitehurst, Fred L. Robinson, Mary Shannon Moore. J. Biol. Chem., Mar 2004; 279: 12840 ? 12847.
2. N Hatano, Y Mori, M Oh-hora. Genes Cells, Nov 2003; 8: 847 - 856.
